| Host: | Goat |
| Applications: | Pep-ELISA/WB |
| Reactivity: | Human/Mouse/Dog |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-RAD9A (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 70kDa band observed in lysates of cell lines A431 and Daudi (calculated MW of 42.5kDa according to NP_004575.1). The observed molecular weight corresponds to earlier findings in |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | RAD9A |
| Gene ID: | 5883 |
| Uniprot ID: | RAD9A_HUMAN |
| Immunogen Region: | C-Term |
| Accession Number: | NP_004575.1 |
| Immunogen Sequence: | QGPSPVLAEDSEGE |
| Function | Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds.endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths.and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. RAD9A possesses 3'->5' double stranded DNA exonuclease activity. |
| Protein Name | Cell Cycle Checkpoint Control Protein Rad9aHrad9Dna Repair Exonuclease Rad9 Homolog A |
| Database Links | Reactome: R-HSA-176187Reactome: R-HSA-5685938Reactome: R-HSA-5693607Reactome: R-HSA-5693616Reactome: R-HSA-6804756Reactome: R-HSA-69473Reactome: R-HSA-9709570 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Cell Cycle Checkpoint Control Protein Rad9a antibodyAnti-Hrad9 antibodyAnti-Dna Repair Exonuclease Rad9 Homolog A antibodyAnti-RAD9A antibody |
Information sourced from Uniprot.org
