| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal antibody anti-DNA repair protein RAD51 homolog 2 (201-250 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000 IHC 1:100-1:300 ELISA 1:40000 IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAD51B |
| Gene ID: | 5890 |
| Uniprot ID: | RA51B_HUMAN |
| Immunogen Region: | 201-250 aa |
| Specificity: | Rad51B Polyclonal Antibody detects endogenous levels of Rad51B protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the human RAD51L1 at the amino acid range 201-250 |
| Post Translational Modifications | Phosphorylated on tyrosine residues by BCR-ABL. |
| Function | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. |
| Protein Name | Dna Repair Protein Rad51 Homolog 2 R51h2 Rad51 Homolog B Rad51b Rad51-Like Protein 1 |
| Database Links | Reactome: R-HSA-5685942 Reactome: R-HSA-5693554 Reactome: R-HSA-5693568 Reactome: R-HSA-5693579 Reactome: R-HSA-5693616 Reactome: R-HSA-9701192 Reactome: R-HSA-9704331 Reactome: R-HSA-9704646 Reactome: R-HSA-9709603 Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair Protein Rad51 Homolog 2 antibody Anti-R51h2 antibody Anti-Rad51 Homolog B antibody Anti-Rad51b antibody Anti-Rad51-Like Protein 1 antibody Anti-RAD51B antibody Anti-RAD51L1 antibody Anti-REC2 antibody |
Information sourced from Uniprot.org