Anti-RAD51B antibody (1-384) (STJ29042)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RAD51B (1-384) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RAD51B |
| Gene ID | 5890 |
| Uniprot ID | RA51B_HUMAN |
| Immunogen Region | 1-384 |
| Immunogen Sequence | MGSKKLKRVGLSQELCDRLS RHQILTCQDFLCLSPLELMK VTGLSYRGVHELLCMVSRAC APKMQTAYGIKAQRSADFSP AFLSTTLSALDEALHGGVAC GSLTEITGPPGCGKTQFCIM MSILATLPTNMGGLEGAVVY IDTESAFSAERLVEIAESRF PRYFNTEEKLLLTSSKVHLY RELTCDEVLQRIESLEEEII SKGIKLVILDSVASVVRKEF DAQLQGNLKERNKFLAREA |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-384 of human RAD51B (NP_598193.2). |
Additional Info
| Tissue Specificity | Expressed in a wide range of tissues. |
| Post Translational Modifications | Phosphorylated on tyrosine residues by BCR-ABL. |
| Function | Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD51 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway. |
| Protein Name | Dna Repair Protein Rad51 Homolog 2R51h2Rad51 Homolog BRad51bRad51-Like Protein 1 |
| Database Links | Reactome: R-HSA-5685942Reactome: R-HSA-5693554Reactome: R-HSA-5693568Reactome: R-HSA-5693579Reactome: R-HSA-5693616Reactome: R-HSA-9701192Reactome: R-HSA-9704331Reactome: R-HSA-9704646Reactome: R-HSA-9709603Reactome: R-HSA-983231 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna Repair Protein Rad51 Homolog 2 antibodyAnti-R51h2 antibodyAnti-Rad51 Homolog B antibodyAnti-Rad51b antibodyAnti-Rad51-Like Protein 1 antibodyAnti-RAD51B antibodyAnti-RAD51L1 antibodyAnti-REC2 antibody |
Information sourced from Uniprot.org