| Host: |
Rabbit |
| Applications: |
IF |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Rad23B (100-200) is suitable for use in Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
RAD23B |
| Gene ID: |
5887 |
| Uniprot ID: |
RD23B_HUMAN |
| Immunogen Region: |
100-200 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 100-200 of human Rad23B (NP_002865.1). |
| Immunogen Sequence: |
TSSTTTTVAQAPTPVPALAP TSTPASITPASATASSEPAP ASAAKQEKPAEKPAETPVAT SPTATDSTSGDSSRSNLFED ATSALVTGQSYENMVTEIMS M |
| Function | Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome. May play a role in endoplasmic reticulum-associated degradation (ERAD) of misfolded glycoproteins by association with PNGase and delivering deglycosylated proteins to the proteasome. Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with CETN2 appears to stabilize XPC. May protect XPC from proteasomal degradation. The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair. In vitro, the XPC:RAD23B dimer is sufficient to initiate NER.it preferentially binds to cisplatin and UV-damaged double-stranded DNA and also binds to a variety of chemically and structurally diverse DNA adducts. XPC:RAD23B contacts DNA both 5' and 3' of a cisplatin lesion with a preference for the 5' side. XPC:RAD23B induces a bend in DNA upon binding. XPC:RAD23B stimulates the activity of DNA glycosylases TDG and SMUG1. |
| Protein Name | Uv Excision Repair Protein Rad23 Homolog BHr23bHhr23bXp-C Repair-Complementing Complex 58 Kda ProteinP58 |
| Database Links | Reactome: R-HSA-532668Reactome: R-HSA-5689877Reactome: R-HSA-5696394Reactome: R-HSA-5696395 |
| Cellular Localisation | NucleusCytoplasmThe Intracellular Distribution Is Cell Cycle DependentLocalized To The Nucleus And The Cytoplasm During G1 PhaseNuclear Levels Decrease During S-PhaseUpon Entering MitosisRelocalizes In The Cytoplasm Without Association With Chromatin |
| Alternative Antibody Names | Anti-Uv Excision Repair Protein Rad23 Homolog B antibodyAnti-Hr23b antibodyAnti-Hhr23b antibodyAnti-Xp-C Repair-Complementing Complex 58 Kda Protein antibodyAnti-P58 antibodyAnti-RAD23B antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
