Anti-Rad18 antibody [R02-5F3] (STJA0036043)
SPECIFICATIONS
ClonalityMonoclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthesized peptide derived from human RAD18
General Information
| Short Description | Rabbit monoclonal anti-Rad18 for use in WB and FC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/FC |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | R02-5F3 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | Affinity Chromatography |
| Dilution Range | WB 1:500-1:1000FC 1:50-1:100 |
| Formulation | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | RAD18 |
| Gene ID | 56852 |
| Uniprot ID | RAD18_HUMAN |
| Immunogen | A synthesized peptide derived from human RAD18 |
Additional Info
| Function | E3 ubiquitin-protein ligase involved in postreplication repair of UV-damaged DNA. Postreplication repair functions in gap-filling of a daughter strand on replication of damaged DNA. Associates to the E2 ubiquitin conjugating enzyme UBE2B to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. Has ssDNA binding activity. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rad18Postreplication Repair Protein Rad18Hhr18Hrad18Ring Finger Protein 73Ring-Type E3 Ubiquitin Transferase Rad18 |
| Database Links | Reactome: R-HSA-110314Reactome: R-HSA-8866654 |
| Cellular Localisation | NucleusCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeAssociates With ChromatinColocalizes With Slf1 In The Nucleus And To CentrosomesRelocalizes With Slf1 To Nuclear Foci In Response To Dna DamageAccumulates With The Slf1-Slf2 And Smc5-Smc6 Complexes At Replication-Coupled Dna Interstrand Repair And Dna Double-Strand Breaks (Dsbs) Sites On Chromatin In A Ubiquitin-Dependent Manner |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rad18 antibodyAnti-Postreplication Repair Protein Rad18 antibodyAnti-Hhr18 antibodyAnti-Hrad18 antibodyAnti-Ring Finger Protein 73 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rad18 antibodyAnti-RAD18 antibodyAnti-RNF73 antibody |
Information sourced from Uniprot.org