• Immunohistochemistry analysis of RABGAP1 in paraffin-embedded Mouse testis using RABGAP1 Rabbit polyclonal antibody (STJ118249) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of RABGAP1 in paraffin-embedded Human colon carcinoma using RABGAP1 Rabbit polyclonal antibody (STJ118249) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using RABGAP1 Rabbit polyclonal antibody (STJ118249) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RABGAP1 antibody (830-1069) (STJ118249)

SKU:
STJ118249

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RABGAP1 (830-1069) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RABGAP1
Gene ID: 23637
Uniprot ID: RBGP1_HUMAN
Immunogen Region: 830-1069
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 830-1069 of human RABGAP1 (NP_036329.3).
Immunogen Sequence: QQEDPIERFERENRRLQEAN MRLEQENDDLAHELVTSKIA LRKDLDNAEEKADALNKELL MTKQKLIDAEEEKRRLEEES AQLKEMCRRELDKAESEIKK NSSIIGDYKQICSQLSERLE KQQTANKVEIEKIRQKVDDC ERCREFFNKEGRVKGISSTK EVLDEDTDEEKETLKNQLRE MELELAQTKLQLVEAECKIQ DLEHHLGLALNEVQAAKKTW FNRTLSSIKTATGVQGKET
Function May act as a GTPase-activating protein of RAB6A. May play a role in microtubule nucleation by centrosome. May participate in a RAB6A-mediated pathway involved in the metaphase-anaphase transition.
Protein Name Rab Gtpase-Activating Protein 1
Gap And Centrosome-Associated Protein
Rab6 Gtpase-Activating Protein Gapcena
Database Links Reactome: R-HSA-8854214
Cellular Localisation Cytoplasm
Cytosol
Cytoskeleton
Microtubule Organizing Center
Centrosome
Predominantly Cytosolic But Also Associated With The Centrosome
Alternative Antibody Names Anti-Rab Gtpase-Activating Protein 1 antibody
Anti-Gap And Centrosome-Associated Protein antibody
Anti-Rab6 Gtpase-Activating Protein Gapcena antibody
Anti-RABGAP1 antibody
Anti-HSPC094 antibody

Information sourced from Uniprot.org

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