| Host: |
Goat |
| Applications: |
Pep-ELISA/WB |
| Reactivity: |
Human/Dog/Cow |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Goat polyclonal antibody anti-RAB8A (C-Term) is suitable for use in ELISA and Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: |
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: |
0.5 mg/mL |
| Dilution Range: |
Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 25kDa band observed in Human Brain (Cerebellum) , Kidney and Heart lysates (calculated MW of 23.7kDa according to NP_005361.2). Recommended concentration: 0.1-0.3µg/ml. |
| Storage Instruction: |
Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: |
RAB8A |
| Gene ID: |
4218 |
| Uniprot ID: |
RAB8A_HUMAN |
| Immunogen Region: |
C-Term |
| Accession Number: |
NP_005361.2 |
| Immunogen Sequence: |
NQGVKITPDQQKR |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi. Together with MYO5B and RAB11A participates in epithelial cell polarization. Also involved in membrane trafficking to the cilium and ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route. Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation. Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins. |
| Protein Name | Ras-Related Protein Rab-8aOncogene C-Mel |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-2565942Reactome: R-HSA-5620912Reactome: R-HSA-5620916Reactome: R-HSA-8854214Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusEndosome MembraneRecycling Endosome MembraneCell ProjectionCiliumCytoplasmic VesiclePhagosomePhagosome MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyMidbodyCilium AxonemeLysosomeColocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma MembraneIn The Gdp-Bound FormPresent In The Perinuclear RegionShows A Polarized Distribution To Distal Regions Of Cell Protrusions In The Gtp-Bound FormColocalizes With Pard3PrkciExoc5OclnPodxl And Rab11a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And LumenogenesisLocalizes To Tubular Recycling EndosomeRecruited To Phagosomes Containing SAureus Or MTuberculosisNon-Phosphorylated Rab8a Predominantly Localized To The Cytoplasm Whereas Phosphorylated Rab8a Localized To The MembraneColocalized With Mical1Graf1/Arhgap26 And Graf2/Arhgap10 On Endosomal TubulesLocalizes To Enlarged Lysosomes Through Lrrk2 Phosphorylation |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-8a antibodyAnti-Oncogene C-Mel antibodyAnti-RAB8A antibodyAnti-MEL antibodyAnti-RAB8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
