| Host: |
Rabbit |
| Applications: |
WB/IHC-F/IHC-P/ICC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit monoclonal antibody anti-Rab8A (171-184 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunofluorescence research applications. |
| Clonality: |
Monoclonal |
| Clone ID: |
R06-8A7 |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
50mM Tris-Glycine (pH7.4) , 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA |
| Purification: |
Affinity Purified |
| Concentration: |
0.3 mg/ml |
| Dilution Range: |
WB 1:500-1:1000IHC 1:50-1:100IF 1:50-1:200 |
| Storage Instruction: |
Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
| Gene Symbol: |
RAB8A |
| Gene ID: |
4218 |
| Uniprot ID: |
RAB8A_HUMAN |
| Immunogen Region: |
171-184 aa |
| Immunogen: |
A synthetic peptide of human RAB8A |
| Post Translational Modifications | Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2. Phosphorylation by LRRK2 is required for localization to stressed lysosomes. |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi. Together with MYO5B and RAB11A participates in epithelial cell polarization. Also involved in membrane trafficking to the cilium and ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy. Participates in the export of a subset of neosynthesized proteins through a Rab8-Rab10-Rab11-dependent endososomal export route. Targeted to and stabilized on stressed lysosomes through LRRK2 phosphorylation. Suppresses stress-induced lysosomal enlargement through EHBP1 and EHNP1L1 effector proteins. |
| Protein Name | Ras-Related Protein Rab-8aOncogene C-Mel |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-2565942Reactome: R-HSA-5620912Reactome: R-HSA-5620916Reactome: R-HSA-8854214Reactome: R-HSA-8873719Reactome: R-HSA-8876198 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideGolgi ApparatusEndosome MembraneRecycling Endosome MembraneCell ProjectionCiliumCytoplasmic VesiclePhagosomePhagosome MembraneCytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeCentrioleCilium Basal BodyMidbodyCilium AxonemeLysosomeColocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma MembraneIn The Gdp-Bound FormPresent In The Perinuclear RegionShows A Polarized Distribution To Distal Regions Of Cell Protrusions In The Gtp-Bound FormColocalizes With Pard3PrkciExoc5OclnPodxl And Rab11a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And LumenogenesisLocalizes To Tubular Recycling EndosomeRecruited To Phagosomes Containing SAureus Or MTuberculosisNon-Phosphorylated Rab8a Predominantly Localized To The Cytoplasm Whereas Phosphorylated Rab8a Localized To The MembraneColocalized With Mical1Graf1/Arhgap26 And Graf2/Arhgap10 On Endosomal TubulesLocalizes To Enlarged Lysosomes Through Lrrk2 Phosphorylation |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-8a antibodyAnti-Oncogene C-Mel antibodyAnti-RAB8A antibodyAnti-MEL antibodyAnti-RAB8 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
