• Western blot analysis of extracts of various cell lines, using RAB8A antibody (STJ119495) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
  • Western blot analysis of extracts of C6 cells, using RAB8A antibody (STJ119495) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
  • Immunofluorescence analysis of U-2 OS cells using RAB8A antibody (STJ119495) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-RAB8A antibody (140-207) (STJ119495)

SKU:
STJ119495

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB8A (140-207) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB8A
Gene ID: 4218
Uniprot ID: RAB8A_HUMAN
Immunogen Region: 140-207
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 140-207 of human RAB8A (NP_005361.2).
Immunogen Sequence: ALDYGIKFMETSAKANINVE NAFFTLARDIKAKMDKKLEG NSPQGSNQGVKITPDQQKRS SFFRCVLL
Post Translational Modifications Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in polarized vesicular trafficking and neurotransmitter release. Together with RAB11A, RAB3IP, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. Regulates the compacted morphology of the Golgi. Together with MYO5B and RAB11A participates in epithelial cell polarization. Also involved in membrane trafficking to the cilium and ciliogenesis. Together with MICALL2, may also regulate adherens junction assembly. May play a role in insulin-induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore play a role in glucose homeostasis. Involved in autophagy.
Protein Name Ras-Related Protein Rab-8a
Oncogene C-Mel
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-2565942
Reactome: R-HSA-5620912
Reactome: R-HSA-5620916
Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8876198
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Golgi Apparatus
Recycling Endosome Membrane
Cell Projection
Cilium
Cytoplasmic Vesicle
Phagosome
Phagosome Membrane
Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Cilium Basal Body
Midbody
Cilium Axoneme
Colocalizes With Optn At The Golgi Complex And In Vesicular Structures Close To The Plasma Membrane
In The Gdp-Bound Form
Present In The Perinuclear Region
Shows A Polarized Distribution To Distal Regions Of Cell Protrusions In The Gtp-Bound Form
Colocalizes With Pard3
Prkci
Exoc5
Ocln
Podxl And Rab11a In Apical Membrane Initiation Sites (Amis) During The Generation Of Apical Surface And Lumenogenesis
Localizes To Tubular Recycling Endosome
Recruited To Phagosomes Containing S
Aureus Or M
Tuberculosis
Non-Phosphorylated Rab8a Predominantly Localizes To The Cytoplasm Whereas Phosphorylated Rab8a Localizes To The Membrane
Alternative Antibody Names Anti-Ras-Related Protein Rab-8a antibody
Anti-Oncogene C-Mel antibody
Anti-RAB8A antibody
Anti-MEL antibody
Anti-RAB8 antibody

Information sourced from Uniprot.org

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