| Host: | Rabbit |
| Applications: | WB/IP/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: | Affinity purification |
| Concentration: | Lot specific |
| Dilution Range: | WB:1:500-1:1000 IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cells ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAB7A |
| Gene ID: | 7879 |
| Uniprot ID: | RAB7A_HUMAN |
| Immunogen Region: | 100-207 |
| Specificity: | A synthetic peptide corresponding to a sequence within amino acids 100-207 of human RAB7 (NP_004628.4). |
| Immunogen Sequence: | DSWRDEFLIQASPRDPENFP FVVLGNKIDLENRQVATKRA QAWCYSKNNIPYFETSAKEA INVEQAFQTIARNALKQETE VELYNEFPEPIKLDKNDRAK ASAESCSC |
| Tissue Specificity | Widely expressed.high expression found in skeletal muscle. |
| Post Translational Modifications | Deubiquitination at Lys-191 and Lys-194 by USP32. Phosphorylated at Ser-72 by LRRK1.phosphorylation is dependent on protein kinase C (PKC) activation of LRRK1. |
| Function | Small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins playing a key role in the regulation of endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway. Required for the exosomal release of SDCBP, CD63 and syndecan. Required for vesicular trafficking and cell surface expression of ACE2. May play a role in PRPH neuronal intermediate filament assembly. |
| Protein Name | Ras-Related Protein Rab-7a |
| Database Links | Reactome: R-HSA-2132295 Reactome: R-HSA-6798695 Reactome: R-HSA-8854214 Reactome: R-HSA-8873719 Reactome: R-HSA-8876198 Reactome: R-HSA-9013148 Reactome: R-HSA-9013149 Reactome: R-HSA-9013404 Reactome: R-HSA-9013405 Reactome: R-HSA-9013406 Reactome: R-HSA-9013407 Reactome: R-HSA-9013408 Reactome: R-HSA-9013409 Reactome: R-HSA-9013423 Reactome: R-HSA-9035034 Reactome: R-HSA-9636383 Reactome: R-HSA-9636569 |
| Cellular Localisation | Cytoplasmic Vesicle Phagosome Membrane Peripheral Membrane Protein Cytoplasmic Side Late Endosome Membrane Lysosome Membrane Melanosome Membrane Autophagosome Membrane Lipid Droplet Endosome Membrane Mitochondrion Membrane Colocalizes With Osbpl1a At The Late Endosome Found In The Ruffled Border (A Late Endosomal-Like Compartment In The Plasma Membrane) Of Bone-Resorbing Osteoclasts Recruited To Phagosomes Containing S Aureus Or Mycobacterium Lipid Droplet Localization Is Increased Upon Adrb2 Stimulation Recruited To Damaged Mitochondria During Mitophagy In A Rimoc1-Dependent Manner |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-7a antibody Anti-RAB7A antibody Anti-RAB7 antibody |
Information sourced from Uniprot.org