| Host: | Rabbit |
| Applications: | WB/IHC/IF/ELISA |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Rabbit polyclonal anti-Ras-related protein Rab-5A (71-120 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:500-1:2000IHC-P 1:100-1:300ELISA 1:20000IF 1:50-200 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | RAB5A |
| Gene ID: | 5868 |
| Uniprot ID: | RAB5A_HUMAN |
| Immunogen Region: | 71-120 aa |
| Specificity: | Rab 5A Polyclonal Antibody detects endogenous levels of Rab 5A protein. |
| Immunogen: | The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A at the amino acid range 71-120 |
| Function | The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes. Contributes to the regulation of filopodia extension. Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan. Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3. |
| Protein Name | Ras-Related Protein Rab-5a |
| Database Links | Reactome: R-HSA-1660499Reactome: R-HSA-8854214Reactome: R-HSA-8856828Reactome: R-HSA-8873719Reactome: R-HSA-8876198Reactome: R-HSA-9636383Reactome: R-HSA-9820960Reactome: R-HSA-983231 |
| Cellular Localisation | Cell MembraneLipid-AnchorCytoplasmic SideEarly Endosome MembraneMelanosomeCytoplasmic VesicleCell ProjectionRuffleMembraneCytoplasmCytosolPhagosome MembraneEndosome MembraneEnriched In Stage I MelanosomesAlternates Between Membrane-Bound And Cytosolic Forms (Probable) |
| Alternative Antibody Names | Anti-Ras-Related Protein Rab-5a antibodyAnti-RAB5A antibodyAnti-RAB5 antibody |
Information sourced from Uniprot.org
