• Western blot analysis of extracts of various cell lines, using RAB4A antibody (STJ28795) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-RAB4A antibody (50-150) (STJ28795)

SKU:
STJ28795

£112.50 - £522.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB4A (50-150) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB4A
Gene ID: 5867
Uniprot ID: RAB4A_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human RAB4A (NP_004569.2).
Immunogen Sequence: FGSKIINVGGKYVKLQIWDT AGQERFRSVTRSYYRGAAGA LLVYDITSRETYNALTNWLT DARMLASQNIVIILCGNKKD LDADREVTFLEASRFAQENE L
Post Translational Modifications Phosphorylated by CDK1 kinase during mitosis. Serotonylation of Gln-72 by TGM2 during activation and aggregation of platelets leads to constitutive activation of GTPase activity.
Function Small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. Involved in protein transport. Plays a role in vesicular traffic. Mediates VEGFR2 endosomal trafficking to enhance VEGFR2 signaling. Acts as a regulator of platelet alpha-granule release during activation and aggregation of platelets.
Protein Name Ras-Related Protein Rab-4a
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1660499
Reactome: R-HSA-8854214
Reactome: R-HSA-8873719
Reactome: R-HSA-8875656
Cellular Localisation Membrane
Peripheral Membrane Protein
Cytoplasm
Early Endosome Membrane
Recycling Endosome Membrane
Generally Associated With Membranes
Cytoplasmic When Phosphorylated By Cdk1
Alternative Antibody Names Anti-Ras-Related Protein Rab-4a antibody
Anti-RAB4A antibody
Anti-RAB4 antibody

Information sourced from Uniprot.org

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