• Flow cytometric analysis of NIH/3T3 cells using Rab 25 monoclonal antibody (green) and negative control (purple).
  • Immunohistochemistry analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) with DAB staining using Rab 25 monoclonal antibody.
  • Immunofluorescence analysis of A549 cells using Rab 25 monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • Western blot analysis using Rab 25 monoclonal antibody against MCF-7 (1) , T47D (2) and GC7901 (3) cell lysate.

Anti-RAB25 antibody [3F12] (STJ98348)

SKU:
STJ98348

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/IHC/IF/FC/ELISA
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Ras-related protein Rab-25 is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry and ELISA research applications.
Clonality: Monoclonal
Clone ID: 3F12
Conjugation: Unconjugated
Isotype: IgG1
Formulation: Liquid in PBS containing 0.03% Sodium Azide, 0.5% BSA, 50% Glycerol.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB25
Gene ID: 57111
Uniprot ID: RAB25_HUMAN
Specificity: Rab 25 Monoclonal Antibody detects endogenous levels of Rab 25 protein.
Immunogen: Purified recombinant fragment of Rab 25 expressed in E. Coli.
Function Involved in the regulation of cell survival. Promotes invasive migration of cells in which it functions to localize and maintain integrin alpha-V/beta-1 at the tips of extending pseudopodia. Involved in the regulation of epithelial morphogenesis through the control of CLDN4 expression and localization at tight junctions. May selectively regulate the apical recycling pathway. Together with MYO5B regulates transcytosis.
Protein Name Ras-Related Protein Rab-25
Catx-8
Database Links Reactome: R-HSA-8873719
Cellular Localisation Cell Membrane
Lipid-Anchor
Cytoplasmic Side
Cell Projection
Pseudopodium Membrane
Cytoplasmic Vesicle
Colocalizes With Integrin Alpha-V/Beta-1 In Vesicles At The Pseudopodial Tips
Alternative Antibody Names Anti-Ras-Related Protein Rab-25 antibody
Anti-Catx-8 antibody
Anti-RAB25 antibody
Anti-CATX8 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance