• Western blot analysis of extracts of various cell lines, using Parvalbumin (PVALB) (PVALB) antibody (STJ11101635) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of paraffin-embedded mouse brain using Parvalbumin (PVALB) (PVALB) rabbit monoclonal antibody (STJ11101635) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using Parvalbumin (PVALB) (PVALB) rabbit monoclonal antibody (STJ11101635) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human appendix using Parvalbumin (PVALB) (PVALB) rabbit monoclonal antibody (STJ11101635) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human kidney using Parvalbumin (PVALB) rabbit monoclonal antibody (STJ11101635) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-PVALB antibody (1-180) [S5MR] (STJ11101635)

SKU:
STJ11101635

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Host: Rabbit
Applications: WB/IHC-P/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Monoclonal
Clone ID: S5MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:1000-1:6000
IHC-P:1:200-1:2000
IF/ICC:1:100-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PVALB
Gene ID: 5816
Uniprot ID: PRVA_HUMAN
Immunogen Region: 1-180
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human Parvalbumin (PVALB) (P20472).
Immunogen Sequence: MSMTDLLNAEDIKKAVGAFS ATDSFDHKKFFQMVGLKKKS ADDVKKVFHMLDKDKSGFIE EDELGFILKGFSPDARDLSA KETKMLMAAGDKDGDGKIGV DEFSTLVAES
Function In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Protein Name Parvalbumin Alpha
Database Links Reactome: R-HSA-8986944
Alternative Antibody Names Anti-Parvalbumin Alpha antibody
Anti-PVALB antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance