• Immunofluorescence analysis of paraffin-embedded Rat cerebellum tissue using Parvalbumin (PVALB) Rabbit polyclonal antibody (STJ115499) at a dilution of 1:100 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining. Perform microwave antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Immunohistochemistry analysis of Parvalbumin (PVALB) in paraffin-embedded human esophageal using Parvalbumin (PVALB) Rabbit polyclonal antibody (STJ115499) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit polyclonal antibody (STJ115499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of various lysates using Parvalbumin (PVALB) Rabbit polyclonal antibody (STJ115499) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PVALB antibody (1-110) (STJ115499)

SKU:
STJ115499

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PVALB (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PVALB
Gene ID: 5816
Uniprot ID: PRVA_HUMAN
Immunogen Region: 1-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human Parvalbumin (Parvalbumin (PVALB) ) (NP_002845.1).
Immunogen Sequence: MSMTDLLNAEDIKKAVGAFS ATDSFDHKKFFQMVGLKKKS ADDVKKVFHMLDKDKSGFIE EDELGFILKGFSPDARDLSA KETKMLMAAGDKDGDGKIGV DEFSTLVAES
Function In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.
Protein Name Parvalbumin Alpha
Database Links Reactome: R-HSA-8986944
Alternative Antibody Names Anti-Parvalbumin Alpha antibody
Anti-PVALB antibody

Information sourced from Uniprot.org

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