Host: | Rabbit |
Applications: | WB/IHC-P/IF/ICC/ELISA |
Reactivity: | Human/Mouse/Rat |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Clonality: | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
Purification: | Affinity purification |
Concentration: | Lot specific |
Dilution Range: | WB:1:500-1:1000 IHC-P:1:50-1:200 IF/ICC:1:50-1:200 ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Gene Symbol: | PTPRC |
Gene ID: | 5788 |
Uniprot ID: | PTPRC_HUMAN |
Immunogen Region: | 194-1306 |
Specificity: | Recombinant fusion protein containing a sequence corresponding to amino acids 194-390 of human CD45 (NP_002829.3). |
Immunogen Sequence: | SDAYLNASETTTLSPSGSAV ISTTTIATTPSKPTCDEKYA NITVDYLYNKETKLFTAKLN VNENVECGNNTCTNNEVHNL TECKNASVSISHNSCTAPDK TLILDVPPGVEKFQLHDCTQ VEKADTTICLKWKNIETFTC DTQNITYRFQCGNMIFDNKE IKLENLEPEHEYKCDSEILY NNHKFTNASKIIKTDFG |
Tissue Specificity | Isoform 1: Detected in thymocytes. Isoform 2: Detected in thymocytes. Isoform 3: Detected in thymocytes. Isoform 4: Not detected in thymocytes. Isoform 5: Detected in thymocytes. Isoform 6: Not detected in thymocytes. Isoform 7: Detected in thymocytes. Isoform 8: Not detected in thymocytes. |
Post Translational Modifications | Heavily N- and O-glycosylated. |
Function | Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. (Microbial infection) Acts as a receptor for human cytomegalovirus protein UL11 and mediates binding of UL11 to T-cells, leading to reduced induction of tyrosine phosphorylation of multiple signaling proteins upon T-cell receptor stimulation and impaired T-cell proliferation. |
Protein Name | Receptor-Type Tyrosine-Protein Phosphatase C Leukocyte Common Antigen L-Ca T200 Cd Antigen Cd45 |
Database Links | Reactome: R-HSA-202427 Reactome: R-HSA-416700 Reactome: R-HSA-6798695 |
Cellular Localisation | Cell Membrane Single-Pass Type I Membrane Protein Membrane Raft Synapse Colocalized With Dpp4 In Membrane Rafts |
Alternative Antibody Names | Anti-Receptor-Type Tyrosine-Protein Phosphatase C antibody Anti-Leukocyte Common Antigen antibody Anti-L-Ca antibody Anti-T200 antibody Anti-Cd Antigen Cd45 antibody Anti-PTPRC antibody Anti-CD45 antibody |
Information sourced from Uniprot.org