Anti-PTCH1 antibody (266-280) (STJ72564)
SPECIFICATIONS
ClonalityPolyclonal
HostGoat
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Goat polyclonal antibody anti-PTCH1 (266-280) is suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry research applications. |
| Applications | Pep-ELISA/WB/IF/IHC |
| Host | Goat |
| Reactivity | Human/Mouse/Rat/Dog/Cow |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 0.5 mg/mL |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range | Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 140kDa band observed in Mouse fetal Kidney lysates (calculated MW of 159kDa according to NP_032983.1). This molecular weight is routinely observed by other sources. Recommended |
| Formulation | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Target Information
| Gene Symbol | PTCH1 |
| Gene ID | 5727 |
| Uniprot ID | PTC1_HUMAN |
| Accession Number | NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1 |
| Immunogen Region | 266-280 |
| Immunogen Sequence | EELKKINYQVDSWEE |
| Specificity | This antibody is expected to recognize all reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1 |
Additional Info
| Post Translational Modifications | Glycosylation is necessary for SHH binding. In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation. |
| Function | Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. |
| Protein Name | Protein Patched Homolog 1PtcPtc1 |
| Database Links | Reactome: R-HSA-373080Reactome: R-HSA-5610787Reactome: R-HSA-5632681Reactome: R-HSA-5632684Reactome: R-HSA-5635838 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Protein Patched Homolog 1 antibodyAnti-Ptc antibodyAnti-Ptc1 antibodyAnti-PTCH1 antibodyAnti-PTCH antibody |
Information sourced from Uniprot.org