| Host: |
Rabbit |
| Applications: |
WB/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PTBP1 (1-100) is suitable for use in Western Blot and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PTBP1 |
| Gene ID: |
5725 |
| Uniprot ID: |
PTBP1_HUMAN |
| Immunogen Region: |
1-100 |
| Immunogen: |
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PTBP1 (NP_114368.1). |
| Immunogen Sequence: |
MDGIVPDIAVGTKRGSDELF STCVTNGPFIMSSNSASAAN GNDSKKFKGDSRSAGVPSRV IHIRKLPIDVTEGEVISLGL PFGKVTNLLMLKGKNQAFIE |
| Function | Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. Binds to polypyrimidine-rich controlling element (PCE) of CFTR and promotes exon skipping of CFTR exon 9, thereby antagonizing TIA1 and its role in exon inclusion of CFTR exon 9. Plays a role in the splicing of pyruvate kinase PKM by binding repressively to a polypyrimidine tract flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation. |
| Protein Name | Polypyrimidine Tract-Binding Protein 1Ptb57 Kda Rna-Binding Protein Pptb-1Heterogeneous Nuclear Ribonucleoprotein IHnrnp I |
| Database Links | Reactome: R-HSA-6803529Reactome: R-HSA-72163Reactome: R-HSA-72203 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Polypyrimidine Tract-Binding Protein 1 antibodyAnti-Ptb antibodyAnti-57 Kda Rna-Binding Protein Pptb-1 antibodyAnti-Heterogeneous Nuclear Ribonucleoprotein I antibodyAnti-Hnrnp I antibodyAnti-PTBP1 antibodyAnti-PTB antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and PTBP1 knockout (KO) HeLa cells, using [KO Validated] PTBP1 Rabbit polyclonal antibody (STJ11100025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/89097/357556/STJ11100025_1__39196.1713121679.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and PTBP1 knockout (KO) HeLa cells, using [KO Validated] PTBP1 Rabbit polyclonal antibody (STJ11100025) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.")