• Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
  • Western blot analysis of lysates from COLO and Jurkat cells, using PRPF19 Antibody. The lane on the right is blocked with the synthesized peptide.
  • Western blot analysis of various cells using PRP19 Polyclonal Antibody

Anti-PRPF19 antibody (171-220 aa) (STJ95229)

SKU:
STJ95229

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pre-mRNA-processing factor 19 (171-220 aa) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRPF19
Gene ID: 27339
Uniprot ID: PRP19_HUMAN
Immunogen Region: 171-220 aa
Specificity: PRP19 Polyclonal Antibody detects endogenous levels of PRP19 protein.
Immunogen: The antiserum was produced against synthesized peptide derived from the human PRPF19 at the amino acid range 171-220
Function Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome.
Protein Name Pre-Mrna-Processing Factor 19
Nuclear Matrix Protein 200
Prp19/Pso4 Homolog
Hpso4
Ring-Type E3 Ubiquitin Transferase Prp19
Senescence Evasion Factor
Database Links Reactome: R-HSA-6781823
Reactome: R-HSA-6781827
Reactome: R-HSA-6782135
Reactome: R-HSA-6782210
Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleoplasm
Cytoplasm
Cytoskeleton
Spindle
Lipid Droplet
Nucleoplasmic In Interphase Cells
Irregularly Distributed In Anaphase Cells
In Prophase Cells
Uniformly Distributed
But Not Associated With Condensing Chromosomes
Found In Extrachromosomal Regions In Metaphase Cells
Mainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During Anaphase
When Nuclei Reform During Late Telophase
Uniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing Chromatin
Recruited On Damaged Dna At Sites Of Double-Strand Break
Alternative Antibody Names Anti-Pre-Mrna-Processing Factor 19 antibody
Anti-Nuclear Matrix Protein 200 antibody
Anti-Prp19/Pso4 Homolog antibody
Anti-Hpso4 antibody
Anti-Ring-Type E3 Ubiquitin Transferase Prp19 antibody
Anti-Senescence Evasion Factor antibody
Anti-PRPF19 antibody
Anti-NMP200 antibody
Anti-PRP19 antibody
Anti-SNEV antibody

Information sourced from Uniprot.org

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