| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRPF19 (1-220) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRPF19 |
| Gene ID: |
27339 |
| Uniprot ID: |
PRP19_HUMAN |
| Immunogen Region: |
1-220 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human PRP19 (NP_055317.1). |
| Immunogen Sequence: |
MSLICSISNEVPEHPCVSPV SNHVYERRLIEKYIAENGTD PINNQPLSEEQLIDIKVAHP IRPKPPSATSIPAILKALQD EWDAVMLHSFTLRQQLQTTR QELSHALYQHDAACRVIARL TKEVTAAREALATLKPQAGL IVPQAVPSSQPSVVGAGEPM DLGELVGMTPEIIQKLQDKA TVLTTERKKRGKTVPEELVK PEELSKYRQVASHVGLHSAS |
| Tissue Specificity | Ubiquitous. Weakly expressed in senescent cells of different tissue origins. Highly expressed in tumor cell lines. |
| Function | Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Required for pre-mRNA splicing as component of the spliceosome. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation. May play a role in the biogenesis of lipid droplets. May play a role in neural differentiation possibly through its function as part of the spliceosome. |
| Protein Name | Pre-Mrna-Processing Factor 19Nuclear Matrix Protein 200Prp19/Pso4 HomologHpso4Ring-Type E3 Ubiquitin Transferase Prp19Senescence Evasion Factor |
| Database Links | Reactome: R-HSA-6781823Reactome: R-HSA-6781827Reactome: R-HSA-6782135Reactome: R-HSA-6782210Reactome: R-HSA-72163 |
| Cellular Localisation | NucleusNucleoplasmCytoplasmCytoskeletonSpindleLipid DropletNucleoplasmic In Interphase CellsIrregularly Distributed In Anaphase CellsIn Prophase CellsUniformly DistributedBut Not Associated With Condensing ChromosomesFound In Extrachromosomal Regions In Metaphase CellsMainly Localized To The Mitotic Spindle Apparatus When Chromosomes Segregate During AnaphaseWhen Nuclei Reform During Late TelophaseUniformly Distributed In Daughter Cells And Displays No Preferred Association With Decondensing ChromatinRecruited On Damaged Dna At Sites Of Double-Strand Break |
| Alternative Antibody Names | Anti-Pre-Mrna-Processing Factor 19 antibodyAnti-Nuclear Matrix Protein 200 antibodyAnti-Prp19/Pso4 Homolog antibodyAnti-Hpso4 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Prp19 antibodyAnti-Senescence Evasion Factor antibodyAnti-PRPF19 antibodyAnti-NMP200 antibodyAnti-PRP19 antibodyAnti-SNEV antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
