| Host: | Goat |
| Applications: | Pep-ELISA/IF |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Goat polyclonal antibody anti-PROM1/CD133 (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
| Clonality : | Polyclonal |
| Conjugation: | Unconjugated |
| Isotype: | IgG |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Concentration: | 0.5 mg/mL |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000. IF: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | PROM1 |
| Gene ID: | 8842 |
| Uniprot ID: | PROM1_HUMAN |
| Immunogen Region: | Internal |
| Accession Number: | NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1 |
| Specificity: | This antibody is expected to recognize all reported isoforms (NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1). Reported variants represent identical protein: NP_001139320.1, NP_001139319.1 |
| Immunogen Sequence: | RHATPTTRGCVSN |
| Post Translational Modifications | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
| Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
| Protein Name | Prominin-1 Antigen Ac133 Prominin-Like Protein 1 Cd Antigen Cd133 |
| Cellular Localisation | Apical Cell Membrane Multi-Pass Membrane Protein Cell Projection Microvillus Membrane Cilium Photoreceptor Outer Segment Endoplasmic Reticulum Endoplasmic Reticulum-Golgi Intermediate Compartment Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid Saliva Seminal Fluid And Urine |
| Alternative Antibody Names | Anti-Prominin-1 antibody Anti-Antigen Ac133 antibody Anti-Prominin-Like Protein 1 antibody Anti-Cd Antigen Cd133 antibody Anti-PROM1 antibody Anti-PROML1 antibody Anti-MSTP061 antibody |
Information sourced from Uniprot.org