Host: | Goat |
Applications: | Pep-ELISA/IF |
Reactivity: | Human |
Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Short Description : | Goat polyclonal antibody anti-PROM1/CD133 (Internal) is suitable for use in ELISA and Immunofluorescence research applications. |
Clonality : | Polyclonal |
Conjugation: | Unconjugated |
Isotype: | IgG |
Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Concentration: | 0.5 mg/mL |
Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:16000.IF: Strong expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 10µg/ml. |
Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
Gene Symbol: | PROM1 |
Gene ID: | 8842 |
Uniprot ID: | PROM1_HUMAN |
Immunogen Region: | Internal |
Accession Number: | NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1 |
Specificity: | This antibody is expected to recognize all reported isoforms (NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1). Reported variants represent identical protein: NP_001139320.1, NP_001139319.1 |
Immunogen Sequence: | RHATPTTRGCVSN |
Post Translational Modifications | Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis. |
Function | May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. |
Protein Name | Prominin-1Antigen Ac133Prominin-Like Protein 1Cd Antigen Cd133 |
Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinCell ProjectionMicrovillus MembraneCiliumPhotoreceptor Outer SegmentEndoplasmic ReticulumEndoplasmic Reticulum-Golgi Intermediate CompartmentFound In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal FluidSalivaSeminal Fluid And Urine |
Alternative Antibody Names | Anti-Prominin-1 antibodyAnti-Antigen Ac133 antibodyAnti-Prominin-Like Protein 1 antibodyAnti-Cd Antigen Cd133 antibodyAnti-PROM1 antibodyAnti-PROML1 antibodyAnti-MSTP061 antibody |
Information sourced from Uniprot.org