• STJ73622 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73622 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
  • STJ73622 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation overnight (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PROM1/CD133 antibody (310-322) (STJ73622)

SKU:
STJ73622-100

£303.50
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Host: Goat
Applications: Pep-ELISA/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PROM1/CD133 (310-322) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: IF-Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. 10µg/ml
ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PROM1
Gene ID: 8842
Uniprot ID: PROM1_HUMAN
Immunogen Region: 310-322
Accession Number: NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1
Specificity: This antibody is expected to recognize all reported isoforms (NP_006008.1; NP_001139319.1; NP_001139324.1; NP_001139323.1; NP_001139322.1; NP_001139321.1). Reported variants represent identical protein: NP_001139320.1, NP_001139319.1
Immunogen Sequence: HPSSETCNSIRLS
Post Translational Modifications Isoform 1 and isoform 2 are glycosylated. Acetylation at Lys-225, Lys-257 and Lys-264 by NAT8 and NAT8B may control PROM1 protein expression and its function in cell apoptosis.
Function May play a role in cell differentiation, proliferation and apoptosis. Binds cholesterol in cholesterol-containing plasma membrane microdomains and may play a role in the organization of the apical plasma membrane in epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner.
Protein Name Prominin-1
Antigen Ac133
Prominin-Like Protein 1
Cd Antigen Cd133
Cellular Localisation Apical Cell Membrane
Multi-Pass Membrane Protein
Cell Projection
Microvillus Membrane
Cilium
Photoreceptor Outer Segment
Endoplasmic Reticulum
Endoplasmic Reticulum-Golgi Intermediate Compartment
Found In Extracellular Membrane Particles In Various Body Fluids Such As Cerebrospinal Fluid
Saliva
Seminal Fluid And Urine
Alternative Antibody Names Anti-Prominin-1 antibody
Anti-Antigen Ac133 antibody
Anti-Prominin-Like Protein 1 antibody
Anti-Cd Antigen Cd133 antibody
Anti-PROM1 antibody
Anti-PROML1 antibody
Anti-MSTP061 antibody

Information sourced from Uniprot.org

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