| Host: | Mouse |
| Applications: | FC/ICC/WB |
| Reactivity: | Human |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Pro-nerve growth factor (Full-Length) for use in FC, ICC and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | BS312 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2b, lambda |
| Formulation: | Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives. |
| Purification: | IgG |
| Dilution Range: | WB 1-2 µg/mLICC 1-2 µg/mL |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives. Store lyophilized antibody at 2-8°C. After reconstitution divide into ali |
| Gene Symbol: | NGF |
| Gene ID: | 4803 |
| Uniprot ID: | NGF_HUMAN |
| Immunogen Region: | Full-Length |
| Specificity: | Human Species cross-reactivity not tested. |
| Immunogen: | A synthetic peptide (C-HTIPQAHWTKLQ, aa: 30-41) of human proNGF protein has been used as the immunogen. The sequence is located on the pro-domain of the proNGF full-length protein and is 80% homologous to mouse and rat proNGF. |
| Immunogen Sequence: | Human |
| Function | Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. Binds lysophosphatidylinositol and lysophosphatidylserine between the two chains of the homodimer. The lipid-bound form promotes histamine relase from mast cells, contrary to the lipid-free form. |
| Protein Name | Beta-Nerve Growth FactorBeta-Ngf |
| Database Links | Reactome: R-HSA-167021Reactome: R-HSA-167044Reactome: R-HSA-167060Reactome: R-HSA-170968Reactome: R-HSA-170984Reactome: R-HSA-177504Reactome: R-HSA-187042Reactome: R-HSA-187706Reactome: R-HSA-193648Reactome: R-HSA-193670Reactome: R-HSA-193681Reactome: R-HSA-198203Reactome: R-HSA-198745Reactome: R-HSA-205017Reactome: R-HSA-205025Reactome: R-HSA-205043Reactome: R-HSA-209543Reactome: R-HSA-209560Reactome: R-HSA-209563 |
| Cellular Localisation | SecretedEndosome LumenProngf Is Endocytosed After Binding To The Cell Surface Receptor Formed By Sort1 And Ngfr |
| Alternative Antibody Names | Anti-Beta-Nerve Growth Factor antibodyAnti-Beta-Ngf antibodyAnti-NGF antibodyAnti-NGFB antibody |
Information sourced from Uniprot.org