| Host: | Mouse |
| Applications: | FC/ICC |
| Reactivity: | Human/Mouse/Rat |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal anti-Pro-glial cell line-derived neurotrophic factor (Full-Length) for use in FC and ICC in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Clonality : | Monoclonal |
| Clone ID : | BS376 |
| Conjugation: | Unconjugated |
| Isotype: | IgG3, kappa |
| Formulation: | Lyophilized from a solution containing PBS buffer pH 7.4 with 3% trehalose, without preservatives. |
| Purification: | IgG |
| Dilution Range: | ICC 1-2 µg/mL |
| Storage Instruction: | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Final buffer contains no preservatives.After reconstitution divide into aliquots and store at-20°C for a higher s |
| Gene Symbol: | GDNF |
| Gene ID: | 2668 |
| Uniprot ID: | GDNF_HUMAN |
| Immunogen Region: | Full-Length |
| Specificity: | Human rat. Antibody is expected to detect mouse proGDNF protein due to 100% amino acid sequence homology. |
| Immunogen: | A synthetic peptide (C-EDYPDQFDDVMD, aa: 55-66) of human proGDNF protein has been used as the immunogen. The sequence is located on the pro-domain of the proGDNF full-length protein and is homologous with mouse and rat form of proGDNF. |
| Immunogen Sequence: | Human |
| Tissue Specificity | In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. Isoform 2 is absent from most tissues except for low levels in intestine and kidney. Highest expression of isoform 3 is found in pancreatic islets. Isoform 5 is expressed at very low levels in putamen, nucleus accumbens, prefrontal cortex, amygdala, hypothalamus and intestine. Isoform 3 is up-regulated in the middle temporal gyrus of Alzheimer disease patients while isoform 2 shows no change. |
| Function | Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. Acts by binding to its coreceptor, GFRA1, leading to autophosphorylation and activation of the RET receptor. Involved in the development of the neural crest. |
| Protein Name | Glial Cell Line-Derived Neurotrophic FactorHgdnfAstrocyte-Derived Trophic FactorAtf |
| Database Links | Reactome: R-HSA-419037Reactome: R-HSA-5673001Reactome: R-HSA-8853659Reactome: R-HSA-9830674 |
| Cellular Localisation | Secreted |
| Alternative Antibody Names | Anti-Glial Cell Line-Derived Neurotrophic Factor antibodyAnti-Hgdnf antibodyAnti-Astrocyte-Derived Trophic Factor antibodyAnti-Atf antibodyAnti-GDNF antibody |
Information sourced from Uniprot.org