• Western blot analysis of extracts of normal 293F cells and 293F transfected with 293F-Prolactin-Avi-His-C protein, using PRL rabbit polyclonal antibody (STJ25144) at 1:700 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
  • Immunohistochemistry analysis of paraffin-embedded Human pituitary gland using PRL rabbit polyclonal antibody (STJ25144) at dilution of 1:600 (40x lens). Perform high pressure antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-PRL antibody (29-227) (STJ25144)

SKU:
STJ25144

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Host: Rabbit
Applications: WB/IHC-P/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:1000
IHC-P:1:500-1:1000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRL
Gene ID: 5617
Uniprot ID: PRL_HUMAN
Immunogen Region: 29-227
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 29-227 of human PRL (NP_001157030.1).
Immunogen Sequence: LPICPGGAARCQVTLRDLFD RAVVLSHYIHNLSSEMFSEF DKRYTHGRGFITKAINSCHT SSLATPEDKEQAQQMNQKDF LSLIVSILRSWNEPLYHLVT EVRGMQEAPEAILSKAVEIE EQTKRLLEGMELIVSQVHPE TKENEIYPVWSGLPSLQMAD EESRLSAYYNLLHCLRRDSH KIDNYLKLLKCRIIHNNNC
Function Prolactin acts primarily on the mammary gland by promoting lactation.
Protein Name Prolactin
Prl
Database Links Reactome: R-HSA-1170546
Reactome: R-HSA-977225
Reactome: R-HSA-982772
Cellular Localisation Secreted
Alternative Antibody Names Anti-Prolactin antibody
Anti-Prl antibody
Anti-PRL antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance