• Immunofluorescence analysis of L929 cells using PRKRIP1 Rabbit polyclonal antibody (STJ119805) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of HeLa cells using PRKRIP1 Rabbit polyclonal antibody (STJ119805) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PRKRIP1 Rabbit polyclonal antibody (STJ119805) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PRKRIP1 Rabbit polyclonal antibody (STJ119805) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PRKRIP1 antibody (67-134) (STJ119805)

SKU:
STJ119805

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRKRIP1 (67-134) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKRIP1
Gene ID: 79706
Uniprot ID: PKRI1_HUMAN
Immunogen Region: 67-134
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 67-134 of human PRKRIP1 (NP_078929.1).
Immunogen Sequence: VMGSSAGAGSGEFHVYRHLR RREYQRQDYMDAMAEKQKLD AEFQKRLEKNKIAAEEQTAK RRKKRQKL
Function Required for pre-mRNA splicing as component of the spliceosome. Binds double-stranded RNA. Inhibits EIF2AK2 kinase activity.
Protein Name Prkr-Interacting Protein 1
Database Links Reactome: R-HSA-72163
Cellular Localisation Nucleus
Nucleolus
Alternative Antibody Names Anti-Prkr-Interacting Protein 1 antibody
Anti-PRKRIP1 antibody

Information sourced from Uniprot.org

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