• Western blot analysis of extracts of various cell lines, using PRKRA/PACTrabbit monoclonal antibody (STJ11102374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry analysis of PRKRA/PACT in paraffin-embedded mouse brain tissue using PRKRA/PACT Rabbit monoclonal antibody (STJ11102374) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded rat brain using PRKRA/PACTrabbit monoclonal antibody (STJ11102374) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRKRA/PACT in paraffin-embedded human brain tissue using PRKRA/PACT Rabbit monoclonal antibody (STJ11102374) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded human brain using PRKRA/PACTrabbit monoclonal antibody (STJ11102374) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of PRKRA/PACT in paraffin-embedded rat brain tissue using PRKRA/PACT Rabbit monoclonal antibody (STJ11102374) at a dilution of 1:400 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry of paraffin-embedded mouse spinal cord using PRKRA/PACTrabbit monoclonal antibody (STJ11102374) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of various lysates using PRKRA/PACTRabbit monoclonal antibody (STJ11102374) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-PRKRA antibody (214-313) [S4MR] (STJ11102374)

SKU:
STJ11102374

£146.50 - £639.50
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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-PRKRA (214-313) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: S4MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKRA
Gene ID: 8575
Uniprot ID: PRKRA_HUMAN
Immunogen Region: 214-313
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 214-313 of human PRKRA/PACT (O75569).
Immunogen Sequence: TWHSLRNSPGEKINLLKRSL LSIPNTDYIQLLSEIAKEQG FNITYLDIDELSANGQYQCL AELSTSPITVCHGSGISCGN AQSDAAHNALQYLKIIAERK
Post Translational Modifications Phosphorylated at Ser-246 in unstressed cells and at Ser-287 in stressed cells. Phosphorylation at Ser-246 appears to be a prerequisite for subsequent phosphorylation at Ser-287. Phosphorylation at Ser-246 and Ser-287 are necessary for activation of EIF2AK2/PKR under conditions of stress.
Function Activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. Required for siRNA production by DICER1 and for subsequent siRNA-mediated post-transcriptional gene silencing. Does not seem to be required for processing of pre-miRNA to miRNA by DICER1. Promotes UBC9-p53/TP53 association and sumoylation and phosphorylation of p53/TP53 at 'Lys-386' at 'Ser-392' respectively and enhances its activity in a EIF2AK2/PKR-dependent manner.
Protein Name Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator A
Pkr-Associated Protein X
Pkr-Associating Protein X
Protein Activator Of The Interferon-Induced Protein Kinase
Protein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator
Database Links Reactome: R-HSA-203927
Reactome: R-HSA-426486
Cellular Localisation Cytoplasm
Perinuclear Region
Alternative Antibody Names Anti-Interferon-Inducible Double-Stranded Rna-Dependent Protein Kinase Activator A antibody
Anti-Pkr-Associated Protein X antibody
Anti-Pkr-Associating Protein X antibody
Anti-Protein Activator Of The Interferon-Induced Protein Kinase antibody
Anti-Protein Kinase - Interferon-Inducible Double-Stranded Rna-Dependent Activator antibody
Anti-PRKRA antibody
Anti-PACT antibody
Anti-RAX antibody
Anti-HSD-14 antibody
Anti-HSD14 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance