| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRKCZ (476-547) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:100-1:500 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRKCZ |
| Gene ID: |
5590 |
| Uniprot ID: |
KPCZ_HUMAN |
| Immunogen Region: |
476-547 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 476-547 of human PKC zeta (NP_002735.3). |
| Immunogen Sequence: |
RIPRFLSVKASHVLKGFLNK DPKERLGCRPQTGFSDIKSH AFFRSIDWDLLEKKQALPPF QPQITDDYGLDN |
| Tissue Specificity | Expressed in brain, and to a lesser extent in lung, kidney and testis. |
| Post Translational Modifications | CDH5 is required for its phosphorylation at Thr-410. Phosphorylated by protein kinase PDPK1.phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-410 by PI3K activates the kinase. |
| Function | Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Phosphorylates VAMP2 in vitro. Isoform 2: Involved in late synaptic long term potention phase in CA1 hippocampal cells and long term memory maintenance. |
| Protein Name | Protein Kinase C Zeta TypeNpkc-Zeta |
| Database Links | Reactome: R-HSA-114604Reactome: R-HSA-2173791Reactome: R-HSA-5218921Reactome: R-HSA-5668599Reactome: R-HSA-9634635 |
| Cellular Localisation | CytoplasmEndosomeCell JunctionMembranePeripheral Membrane ProteinIn The RetinaLocalizes In The Terminals Of The Rod Bipolar CellsAssociates With EndosomesPresence Of Krit1Cdh5 And Rap1b Is Required For Its Localization To The Cell JunctionColocalizes With Vamp2 And Wdfy2 In Intracellular VesiclesTransiently Translocates To The Membrane Of Ca1 Hippocampal Cells In Response To The Induction Of Long Term PotentiationIsoform 2: Cytoplasm |
| Alternative Antibody Names | Anti-Protein Kinase C Zeta Type antibodyAnti-Npkc-Zeta antibodyAnti-PRKCZ antibodyAnti-PKC2 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
