Anti-PRKCA antibody [M499] (STJA0005532)

SKU:
STJA0005532-100

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Host: Mouse
Applications: WB/ICC
Reactivity: Chicken/Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-PKC is suitable for use in Western Blot and Immunocytochemistry research applications.
Clonality: Monoclonal
Clone ID: M499
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Purification: Antigen Affinity Purified
Dilution Range: WB 1:1000
IHC
ICC 1:100
IP
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKCG
Gene ID: 5582
Uniprot ID: KPCG_HUMAN
Specificity: This antibody detects 80-82kDa* proteins corresponding to the molecular mass of PKC Alpha, PKC Beta, and PKC Gamma on SDS-PAGE immunoblots of mouse brain lysates. Similar results were observed in human and mouse lysates. This antibody detects recombi
Immunogen: Clone (M499) was generated from a recombinant human PKC Gamma that included amino acids residues 499-697.
Post Translational Modifications Autophosphorylation on Thr-674 appears to regulate motor functions of junctophilins, JPH3 and JPH4. Ubiquitinated.
Function Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GRIA4/GLUR4 and GRIN1/NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GRIA4/GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor GRM5/MGLUR5 and phosphorylates GRIN1/NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May be involved in desensitization of mu-type opioid receptor-mediated G-protein activation in the spinal cord, and may be critical for the development and/or maintenance of morphine-induced reinforcing effects in the limbic forebrain. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43 (GJA1/CX43), resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53/TP53 and promotes p53/TP53-dependent apoptosis in response to DNA damage. Involved in the phase resetting of the cerebral cortex circadian clock during temporally restricted feeding. Stabilizes the core clock component BMAL1 by interfering with its ubiquitination, thus suppressing its degradation, resulting in phase resetting of the cerebral cortex clock.
Protein Name Protein Kinase C Gamma Type
Pkc-Gamma
Database Links Reactome: R-HSA-111933
Reactome: R-HSA-114516
Reactome: R-HSA-416993
Reactome: R-HSA-418597
Reactome: R-HSA-5099900
Reactome: R-HSA-76005
Cellular Localisation Cytoplasm
Perinuclear Region
Cell Membrane
Peripheral Membrane Protein
Synapse
Synaptosome
Cell Projection
Dendrite
Translocates To Synaptic Membranes On Stimulation
Alternative Antibody Names Anti-Protein Kinase C Gamma Type antibody
Anti-Pkc-Gamma antibody
Anti-PRKCG antibody
Anti-PKCG antibody

Information sourced from Uniprot.org

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