Anti-PRKAG1 antibody [5H5] (STJ196661)
SPECIFICATIONS
ClonalityMonoclonal
HostMouse
ConjugationUnconjugated
ImmunogenSynthesized peptide derived from the human AMPK Gamma 1
General Information
| Short Description | Mouse monoclonal anti-5 AMP-activated protein kinase subunit gamma-1 for use in IHC and WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Mouse |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal |
| Clone ID | 5H5 |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. |
| Dilution Range | IHC-P 1:50-200WB 1:1000-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRKAG1 |
| Gene ID | 5571 |
| Uniprot ID | AAKG1_HUMAN |
| Immunogen | Synthesized peptide derived from the human AMPK Gamma 1 |
| Specificity | This antibody detects endogenous levels of AMPK Gamma 1 at Human, Mouse, Rat |
Additional Info
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Gamma-1Ampk Gamma1Ampk Subunit Gamma-1Ampkg |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Cellular Localisation | |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Gamma-1 antibodyAnti-Ampk Gamma1 antibodyAnti-Ampk Subunit Gamma-1 antibodyAnti-Ampkg antibodyAnti-PRKAG1 antibody |
Information sourced from Uniprot.org