| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-PRKAG1 (1-331) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:100 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PRKAG1 |
| Gene ID: |
5571 |
| Uniprot ID: |
AAKG1_HUMAN |
| Immunogen Region: |
1-331 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human PRKAG1 (NP_002724.1). |
| Immunogen Sequence: |
METVISSDSSPAVENEHPQE TPESNNSVYTSFMKSHRCYD LIPTSSKLVVFDTSLQVKKA FFALVTNGVRAAPLWDSKKQ SFVGMLTITDFINILHRYYK SALVQIYELEEHKIETWREV YLQDSFKPLVCISPNASLFD AVSSLIRNKIHRLPVIDPES GNTLYILTHKRILKFLKLFI TEFPKPEFMSKSLEELQIGT YANIAMVRTTTPVYVALGIF VQHRVSALPVVDEKGRVVD |
| Post Translational Modifications | Phosphorylated by ULK1 and ULK2.leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1, ULK2 and AMPK. |
| Function | AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Gamma-1Ampk Gamma1Ampk Subunit Gamma-1Ampkg |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Gamma-1 antibodyAnti-Ampk Gamma1 antibodyAnti-Ampk Subunit Gamma-1 antibodyAnti-Ampkg antibodyAnti-PRKAG1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
