| Host: | Mouse |
| Applications: | WB/ICC/IP/IHC |
| Reactivity: | Human/Mouse/Rat/Monkey |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description : | Mouse monoclonal antibody anti-5 AMP-activated protein kinase subunit beta-1 is suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry research applications. |
| Clonality : | Monoclonal |
| Clone ID : | 1A7-E11-E9 |
| Conjugation: | Unconjugated |
| Isotype: | IgG2a |
| Formulation: | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Purification: | The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen. |
| Concentration: | 1 mg/mL |
| Dilution Range: | WB 1:1000ICC 1:100 |
| Storage Instruction: | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: | PRKAB1 |
| Gene ID: | 5564 |
| Uniprot ID: | AAKB1_HUMAN |
| Specificity: | This antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins. |
| Immunogen: | Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli. |
| Function | Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). |
| Protein Name | 5'-Amp-Activated Protein Kinase Subunit Beta-1Ampk Subunit Beta-1Ampkb |
| Database Links | Reactome: R-HSA-1445148Reactome: R-HSA-1632852Reactome: R-HSA-2151209Reactome: R-HSA-380972Reactome: R-HSA-5628897Reactome: R-HSA-6804756Reactome: R-HSA-9613354Reactome: R-HSA-9619483 |
| Alternative Antibody Names | Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibodyAnti-Ampk Subunit Beta-1 antibodyAnti-Ampkb antibodyAnti-PRKAB1 antibodyAnti-AMPK antibody |
Information sourced from Uniprot.org
![Standard Curve for Human Cystatin C: Capture Antibody Mouse mAb [6F12-C7-D8] to Human Cystatin C at 4 Mu g/ml and Detector Antibody Mouse mAb [200912-5C3:5C3-C7.200912-3B11:3B11-E9]to Human Cystatin C at 0.5 Mu g/ml.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/600x533/products/121333/232245/STJ99193_1_ELISA__82703.1602855625.jpg?c=1 "Standard Curve for Human Cystatin C: Capture Antibody Mouse mAb [6F12-C7-D8] to Human Cystatin C at 4 Mu g/ml and Detector Antibody Mouse mAb [200912-5C3:5C3-C7.200912-3B11:3B11-E9]to Human Cystatin C at 0.5 Mu g/ml.")
