• Immunohistochemical analysis of paraffin-embedded Breast cancer using AMPK beta 1 mouse mAb (1/200 dilution).Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).
  • Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using AMPK beta 1 mouse mAb (dilution 1:100).
  • Immunoprecipitation analysis of Hela cell lysates using AMPK beta 1 mouse mAb.
  • Western blot detection of AMPK beta 1 in 3T3, Hela, PC-12, COS7 and MDA-MB-468 cell lysates using AMPK beta 1 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa.Exposure time:5min.

Anti-PRKAB1 antibody [1A7-E11-E9] (STJ99185)

SKU:
STJ99185

£139.00 - £336.00
Current Stock:
Host: Mouse
Applications: WB/ICC/IP/IHC
Reactivity: Human/Mouse/Rat/Monkey
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-5 AMP-activated protein kinase subunit beta-1 is suitable for use in Western Blot, Immunocytochemistry, Immunoprecipitation and Immunohistochemistry research applications.
Clonality: Monoclonal
Clone ID: 1A7-E11-E9
Conjugation: Unconjugated
Isotype: IgG2a
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:1000
ICC 1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAB1
Gene ID: 5564
Uniprot ID: AAKB1_HUMAN
Specificity: This antibody detects endogenous levels of AMPK beta 1 and does not cross-react with related proteins.
Immunogen: Purified recombinant human AMPK beta 1 protein fragments expressed in E.coli.
Function Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton.probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).
Protein Name 5'-Amp-Activated Protein Kinase Subunit Beta-1
Ampk Subunit Beta-1
Ampkb
Database Links Reactome: R-HSA-1445148
Reactome: R-HSA-1632852
Reactome: R-HSA-2151209
Reactome: R-HSA-380972
Reactome: R-HSA-5628897
Reactome: R-HSA-6804756
Reactome: R-HSA-9613354
Reactome: R-HSA-9619483
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Subunit Beta-1 antibody
Anti-Ampk Subunit Beta-1 antibody
Anti-Ampkb antibody
Anti-PRKAB1 antibody
Anti-AMPK antibody

Information sourced from Uniprot.org

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