• Immunofluorescence analysis of U2OS cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ119425) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of PC-12 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ119425) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit polyclonal antibody (STJ119425) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of 293F, using AMPKa1/AMPKa2 antibody (STJ119425) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-PRKAA1/PRKAA2 antibody (5-280) (STJ119425)

SKU:
STJ119425

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Host: Rabbit
Applications: WB/IF/ICC/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:100-1:500
IF/ICC:1:50-1:200
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRKAA2
PRKAA1
Gene ID: 5563
5562
Uniprot ID: AAPK2_HUMAN
AAPK1_HUMAN
Immunogen Region: 5-280
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 5-280 of human AMPKa1/AMPKa2 (NP_006242.5).
Immunogen Sequence: SSWRKMATAEKQKHDGRVKI GHYILGDTLGVGTFGKVKVG KHELTGHKVAVKILNRQKIR SLDVVGKIRREIQNLKLFRH PHIIKLYQVISTPSDIFMVM EYVSGGELFDYICKNGRLDE KESRRLFQQILSGVDYCHRH MVVHRDLKPENVLLDAHMNA KIADFGLSNMMSDGEFLRTS CGSPNYAAPEVISGRLYAGP EVDIWSSGVILYALLCGTLP FDDDHVPTLFKKICDGIFY
Tissue Specificity
Alternative Antibody Names Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-2 antibody
Anti-Ampk Subunit Alpha-2 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-PRKAA2 antibody
Anti-AMPK antibody
Anti-AMPK2 antibody
Anti-5'-Amp-Activated Protein Kinase Catalytic Subunit Alpha-1 antibody
Anti-Ampk Subunit Alpha-1 antibody
Anti-Acetyl-Coa Carboxylase Kinase antibody
Anti-Acaca Kinase antibody
Anti-Hydroxymethylglutaryl-Coa Reductase Kinase antibody
Anti-Hmgcr Kinase antibody
Anti-Tau-Protein Kinase Prkaa1 antibody
Anti-PRKAA1 antibody
Anti-AMPK1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance