• Immunofluorescence analysis of L929 cells using PREPL Rabbit polyclonal antibody (STJ116560) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of C6 cells using PREPL Rabbit polyclonal antibody (STJ116560) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using PREPL Rabbit polyclonal antibody (STJ116560) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-PREPL antibody (578-727) (STJ116560)

SKU:
STJ116560

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PREPL (578-727) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PREPL
Gene ID: 9581
Uniprot ID: PPCEL_HUMAN
Immunogen Region: 578-727
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 578-727 of human PREPL (NP_006027.2).
Immunogen Sequence: AVTLEAPFLDVLNTMMDTTL PLTLEELEEWGNPSSDEKHK NYIKRYCPYQNIKPQHYPSI HITAYENDERVPLKGIVSYT EKLKEAIAEHAKDTGEGYQT PNIILDIQPGGNHVIEDSHK KITAQIKFLYEELGLDSTSV FEDLKKYLKF
Tissue Specificity Expressed in pyramidal neurons of the temporal cortex and neocortex (at protein level). Widely expressed. Expressed at higher level in brain, skeletal muscle, heart and kidney. Expressed at the endplates in the neuromuscular junction.
Function Serine peptidase whose precise substrate specificity remains unclear. Does not cleave peptides after a arginine or lysine residue. Regulates trans-Golgi network morphology and sorting by regulating the membrane binding of the AP-1 complex. May play a role in the regulation of synaptic vesicle exocytosis.
Protein Name Prolyl Endopeptidase-Like
Prolylendopeptidase-Like
Cellular Localisation Cytoplasm
Cytosol
Golgi Apparatus
Trans-Golgi Network
Cytoskeleton
Nucleus
Co-Localizes With Ap-1 In The Trans-Golgi Network
Co-Localizes With Map2 And Actb On The Cytoskeleton
Co-Localizes With Stx6 And Gosr2 At The Golgi Apparatus
Alternative Antibody Names Anti-Prolyl Endopeptidase-Like antibody
Anti-Prolylendopeptidase-Like antibody
Anti-PREPL antibody
Anti-KIAA0436 antibody

Information sourced from Uniprot.org

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