Anti-PRDX6 antibody (1-224) (STJ25108)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PRDX6 (1-224) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PRDX6 |
| Gene ID | 9588 |
| Uniprot ID | PRDX6_HUMAN |
| Immunogen Region | 1-224 |
| Immunogen Sequence | MPGGLLLGDVAPNFEANTTV GRIRFHDFLGDSWGILFSHP RDFTPVCTTELGRAAKLAPE FAKRNVKLIALSIDSVEDHL AWSKDINAYNCEEPTEKLPF PIIDDRNRELAILLGMLDPA EKDEKGMPVTARVVFVFGPD KKLKLSILYPATTGRNFDEI LRVVISLQLTAEKRVATPVD WKDGDSVMVLPTIPEEEAKK LFPKGVFTKELPSGKKYLRY TPQP |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human Peroxiredoxin 6 (NP_004896.1). |
Additional Info
| Post Translational Modifications | Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress. Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme. The phosphorylated form exhibits a greater lysophosphatidylcholine acyltransferase activity compared to the non-phosphorylated form. |
| Function | Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. Also has phospholipase activity, can therefore either reduce the oxidized sn-2 fatty acyl group of phospholipids (peroxidase activity) or hydrolyze the sn-2 ester bond of phospholipids (phospholipase activity). These activities are dependent on binding to phospholipids at acidic pH and to oxidized phospholipds at cytosolic pH. Plays a role in cell protection against oxidative stress by detoxifying peroxides and in phospholipid homeostasis. Exhibits acyl-CoA-dependent lysophospholipid acyltransferase which mediates the conversion of lysophosphatidylcholine (1-acyl-sn-glycero-3-phosphocholine or LPC) into phosphatidylcholine (1,2-diacyl-sn-glycero-3-phosphocholine or PC). Shows a clear preference for LPC as the lysophospholipid and for palmitoyl CoA as the fatty acyl substrate. |
| Protein Name | Peroxiredoxin-61-Cys Peroxiredoxin1-Cys Prx24 Kda ProteinAcidic Calcium-Independent Phospholipase A2Aipla2Antioxidant Protein 2Glutathione-Dependent PeroxiredoxinLiver 2d Page Spot 40Lysophosphatidylcholine Acyltransferase 5Lpc Acyltransferase 5Lpcat-5Lyso-Pc Acyltransferase 5Non-Selenium Glutathione PeroxidaseNsgpxRed Blood Cells Page Spot 12 |
| Database Links | Reactome: R-HSA-3299685Reactome: R-HSA-6798695 |
| Cellular Localisation | CytoplasmLysosomeAlso Found In Lung Secretory Organelles (Lamellar Bodies) |
| Alternative Antibody Names | Anti-Peroxiredoxin-6 antibodyAnti-1-Cys Peroxiredoxin antibodyAnti-1-Cys Prx antibodyAnti-24 Kda Protein antibodyAnti-Acidic Calcium-Independent Phospholipase A2 antibodyAnti-Aipla2 antibodyAnti-Antioxidant Protein 2 antibodyAnti-Glutathione-Dependent Peroxiredoxin antibodyAnti-Liver 2d Page Spot 40 antibodyAnti-Lysophosphatidylcholine Acyltransferase 5 antibodyAnti-Lpc Acyltransferase 5 antibodyAnti-Lpcat-5 antibodyAnti-Lyso-Pc Acyltransferase 5 antibodyAnti-Non-Selenium Glutathione Peroxidase antibodyAnti-Nsgpx antibodyAnti-Red Blood Cells Page Spot 12 antibodyAnti-PRDX6 antibodyAnti-AOP2 antibodyAnti-KIAA0106 antibody |
Information sourced from Uniprot.org