• Western blot analysis of extracts from normal (control) and Peroxiredoxin 2 (Peroxiredoxin 2 (PRDX2) ) knockout (KO) 293T cells, using Peroxiredoxin 2 (Peroxiredoxin 2 (PRDX2) ) antibody (STJ25103) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-PRDX2 antibody (1-142) (STJ25103)

SKU:
STJ25103

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PRDX2 (1-142) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PRDX2
Gene ID: 7001
Uniprot ID: PRDX2_HUMAN
Immunogen Region: 1-142
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human Peroxiredoxin 2 (Peroxiredoxin 2 (PRDX2) ) (NP_859428.1).
Immunogen Sequence: MASGNARIGKPAPDFKATAV VDGAFKEVKLSDYKGKYVVL FFYPLDFTFVCPTEIIAFSN RAEDFRKLGCEVLGVSVDSQ FTHLAWYEQGPKREVAAKLT PSGPSSVASWPLLNLWNLRF PIVKIMETLPPKSLRMMTVI SI
Post Translational Modifications The enzyme can be inactivated by further oxidation of the cysteine sulfenic acid (C(P)-SOH) to sulphinic acid (C(P)-SO2H) instead of its condensation to a disulfide bond. It can be reactivated by forming a transient disulfide bond with sulfiredoxin SRXN1, which reduces the cysteine sulfinic acid in an ATP- and Mg-dependent manner.
Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Protein Name Peroxiredoxin-2
Natural Killer Cell-Enhancing Factor B
Nkef-B
Prp
Thiol-Specific Antioxidant Protein
Tsa
Thioredoxin Peroxidase 1
Thioredoxin-Dependent Peroxide Reductase 1
Thioredoxin-Dependent Peroxiredoxin 2
Database Links Reactome: R-HSA-3299685
Reactome: R-HSA-5628897
Reactome: R-HSA-8862803
Cellular Localisation Cytoplasm
Alternative Antibody Names Anti-Peroxiredoxin-2 antibody
Anti-Natural Killer Cell-Enhancing Factor B antibody
Anti-Nkef-B antibody
Anti-Prp antibody
Anti-Thiol-Specific Antioxidant Protein antibody
Anti-Tsa antibody
Anti-Thioredoxin Peroxidase 1 antibody
Anti-Thioredoxin-Dependent Peroxide Reductase 1 antibody
Anti-Thioredoxin-Dependent Peroxiredoxin 2 antibody
Anti-PRDX2 antibody
Anti-NKEFB antibody
Anti-TDPX1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance