• Immunofluorescence analysis of U-2 OS cells using PPP4R3B Rabbit polyclonal antibody (STJ11102873) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of lysates from Mouse testis, using PPP4R3B Rabbit polyclonal antibody (STJ11102873) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-PPP4R3B antibody (628-817) (STJ11102873)

SKU:
STJ11102873

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP4R3B (628-817) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP4R3B
Gene ID: 57223
Uniprot ID: P4R3B_HUMAN
Immunogen Region: 628-817
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 628-817 of human PPP4R3B (NP_001269779.1).
Immunogen Sequence: YKALESIEYVQTFKGLKTKY EQEKDRQNQKLNSVPSILRS NRFRRDAKALEEDEEMWFNE DEEEEGKAVVAPVEKPKPED DFPDNYEKFMETKKAKESED KENLPKRTSPGGFKFTFSHS ASAANGTNSKSVVAQIPPAT SNGSSSKTTNLPTSVTATKG SLVGLVDYPDDEEEDEEEES SPRKRPRLGS
Tissue Specificity Moderately expressed in tissues and specific brain regions examined.
Function Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers.
Protein Name Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 3b
Smek Homolog 2
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Nucleus
In Interphase Localized In The Cytoplasm And (With Higher Levels) The Nucleus
During Metaphase Located In Pericentriolar Regions
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 4 Regulatory Subunit 3b antibody
Anti-Smek Homolog 2 antibody
Anti-PPP4R3B antibody
Anti-KIAA1387 antibody
Anti-PP4R3B antibody
Anti-SMEK2 antibody

Information sourced from Uniprot.org

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