• Western blot analysis of lysates from wild type (WT) and PP2A-Abeta/PR65 Beta/PP2A-A Beta/PR65 Beta/PPP2R1B knockout (KO) 293T cells, using [KO Validated] PP2A-A Beta/PR65 Beta/PPP2R1B Rabbit polyclonal antibody (STJ11100062) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PPP2R1B antibody (468-667) (STJ11100062)

SKU:
STJ11100062

£129.50 - £639.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PPP2R1B (468-667) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPP2R1B
Gene ID: 5519
Uniprot ID: 2AAB_HUMAN
Immunogen Region: 468-667
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 468-667 of human PP2A-Abeta/PR65 Beta/PP2A-A Beta/PR65 Beta/PPP2R1B (NP_859050.1).
Immunogen Sequence: YAIREAATNNLMKLVQKFGT EWAQNTIVPKVLVMANDPNY LHRMTTLFCINALSEACGQE ITTKQMLPIVLKMAGDQVAN VRFNVAKSLQKIGPILDTNA LQGEVKPVLQKLGQDEDMDV KYFAQEAISVVAQRLRKLEF PVKDSGEPSVPRADKNHFPR PTVPGEDMGKGPVYQLRGDT RDTLAQLGIAELVHFSQSTD
Function The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit.
Protein Name Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Beta Isoform
Pp2a Subunit A Isoform Pr65-Beta
Pp2a Subunit A Isoform R1-Beta
Database Links Reactome: R-HSA-113501
Reactome: R-HSA-141444
Reactome: R-HSA-163685
Reactome: R-HSA-163767
Reactome: R-HSA-180024
Reactome: R-HSA-195253
Reactome: R-HSA-196299
Reactome: R-HSA-198753
Reactome: R-HSA-202670
Reactome: R-HSA-2465910
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-389513
Reactome: R-HSA-432142
Reactome: R-HSA-4641262
Reactome: R-HSA-5339716
Reactome: R-HSA-5358747
Reactome: R-HSA-5358749
Reactome: R-HSA-5358751
Reactome: R-HSA-5358752
Reactome: R-HSA-5467337
Reactome: R-HSA-5467340
Reactome: R-HSA-5467348
Reactome: R-HSA-5663220
Reactome: R-HSA-5673000
Reactome: R-HSA-5675221
Reactome: R-HSA-6804757
Reactome: R-HSA-6811558
Reactome: R-HSA-68877
Reactome: R-HSA-69231
Reactome: R-HSA-69273
Reactome: R-HSA-9634600
Reactome: R-HSA-9648025
Alternative Antibody Names Anti-Serine/Threonine-Protein Phosphatase 2a 65 Kda Regulatory Subunit A Beta Isoform antibody
Anti-Pp2a Subunit A Isoform Pr65-Beta antibody
Anti-Pp2a Subunit A Isoform R1-Beta antibody
Anti-PPP2R1B antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance