• STJ70012 (0. 3µg/ml) staining of HepG2 (A) and negative control KLY (B) lysate (35µg protein in RIPA buffer) Detected by chemiluminescence.
  • STJ70012 (2µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
  • STJ70012 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70012 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml) , showing strong nuclear and some cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
  • STJ70012 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (0. 4ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-PPP1R15A/GADD34 antibody (C-Term) (STJ70012)

SKU:
STJ70012-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB/IHC/IF/FC
Reactivity: Human
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-PPP1R15A/GADD34 (C-Term) is suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: WB-0.3-1µg/ml
IHC-2-4µg/ml
IF-Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. 10µg/ml
ELISA-antibody detection limit dilution 1:128000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: PPP1R15A
Gene ID: 23645
Uniprot ID: PR15A_HUMAN
Immunogen Region: C-Term
Accession Number: NP_055145.3
Immunogen Sequence: AAALDLSGRRG
Function Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Acts also a viral restriction factor by attenuating HIV-1 replication. Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR.
Protein Name Protein Phosphatase 1 Regulatory Subunit 15a
Growth Arrest And Dna Damage-Inducible Protein Gadd34
Myeloid Differentiation Primary Response Protein Myd116 Homolog
Database Links Reactome: R-HSA-2173788
Reactome: R-HSA-9648895
Cellular Localisation Endoplasmic Reticulum Membrane
Peripheral Membrane Protein
Cytoplasmic Side
Mitochondrion Outer Membrane
Associates With Membranes Via An N-Terminal Amphipathic Intramembrane Region
Alternative Antibody Names Anti-Protein Phosphatase 1 Regulatory Subunit 15a antibody
Anti-Growth Arrest And Dna Damage-Inducible Protein Gadd34 antibody
Anti-Myeloid Differentiation Primary Response Protein Myd116 Homolog antibody
Anti-PPP1R15A antibody
Anti-GADD34 antibody

Information sourced from Uniprot.org

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