| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Purification: |
Affinity purification |
| Concentration: |
Lot specific |
| Dilution Range: |
WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
PPM1K |
| Gene ID: |
152926 |
| Uniprot ID: |
PPM1K_HUMAN |
| Immunogen Region: |
89-372 |
| Specificity: |
Recombinant fusion protein containing a sequence corresponding to amino acids 89-351 of human PPM1K (NP_689755.3). |
| Immunogen Sequence: |
KISLENVGCASQIGKRKENE DRFDFAQLTDEVLYFAVYDG HGGPAAADFCHTHMEKCIMD LLPKEKNLETLLTLAFLEID KAFSSHARLSADATLLTSGT TATVALLRDGIELVVASVGD SRAILCRKGKPMKLTIDHTP ERKDEKERIKKCGGFVAWNS LGQPHVNGRLAMTRSIGDLD LKTSGVIAEPETKRIKLHHA DDSFLVLTTDGINFMVNSQE ICDFVNQCHDPNEAAHAVT |
| Function | Serine/threonine-protein phosphatase component of macronutrients metabolism. Forms a functional kinase and phosphatase pair with BCKDK, serving as a metabolic regulatory node that coordinates branched-chain amino acids (BCAAs) with glucose and lipid metabolism via two distinct phosphoprotein targets: mitochondrial BCKDHA subunit of the branched-chain alpha-ketoacid dehydrogenase (BCKDH) complex and cytosolic ACLY, a lipogenic enzyme of Krebs cycle. At high levels of branched-chain ketoacids, dephosphorylates and activates mitochondrial BCKDH complex, a multisubunit complex consisting of three multimeric components each involved in different steps of BCAA catabolism: E1 composed of BCKDHA and BCKDHB, E2 core composed of DBT monomers, and E3 composed of DLD monomers. Tightly associates with the E2 component of BCKDH complex and dephosphorylates BCKDHA on Ser-337. Regulates the reversible phosphorylation of ACLY in response to changes in cellular carbohydrate abundance such as occurs during fasting to feeding metabolic transition. At fasting state, appears to dephosphorylate ACLY on Ser-455 and inactivate it. Refeeding stimulates MLXIPL/ChREBP transcription factor, leading to increased BCKDK to PPM1K expression ratio, phosphorylation and activation of ACLY that ultimately results in the generation of malonyl-CoA and oxaloacetate immediate substrates of de novo lipogenesis and gluconeogenesis, respectively. Recognizes phosphosites having SxS or RxxS motifs and strictly depends on Mn(2+) ions for the phosphatase activity. Regulates Ca(2+)-induced opening of mitochondrial transition pore and apoptotic cell death. |
| Protein Name | Protein Phosphatase Mn(2+-Dependent 1kBranched-Chain Alpha-Ketoacid Dehydrogenase PhosphataseBckdhBdpPp2c Domain-Containing Protein Phosphatase 1kPp2c-Like Mitochondrial ProteinPp2c-Type Mitochondrial Phosphoprotein PhosphatasePtmpProtein Phosphatase 2c Family MemberProtein Phosphatase 2c Isoform KappaPp2c-Kappa3-Methyl-2-Oxobutanoate Dehydrogenase2-Methylpropanoyl-Transferring-Phosphatase - Mitochondrial |
| Database Links | Reactome: R-HSA-70895 |
| Cellular Localisation | Mitochondrion MatrixDetected In The Cytosolic Compartment Of Liver Cells |
| Alternative Antibody Names | Anti-Protein Phosphatase Mn(2+-Dependent 1k antibodyAnti-Branched-Chain Alpha-Ketoacid Dehydrogenase Phosphatase antibodyAnti-Bckdh antibodyAnti-Bdp antibodyAnti-Pp2c Domain-Containing Protein Phosphatase 1k antibodyAnti-Pp2c-Like Mitochondrial Protein antibodyAnti-Pp2c-Type Mitochondrial Phosphoprotein Phosphatase antibodyAnti-Ptmp antibodyAnti-Protein Phosphatase 2c Family Member antibodyAnti-Protein Phosphatase 2c Isoform Kappa antibodyAnti-Pp2c-Kappa antibodyAnti-3-Methyl-2-Oxobutanoate Dehydrogenase antibodyAnti-2-Methylpropanoyl-Transferring-Phosphatase - Mitochondrial antibodyAnti-PPM1K antibodyAnti-PP2CM antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
