Western blot analysis of various lysates using PPIH Rabbit polyclonal antibody (STJ114065) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PPIH antibody (1-177) (STJ114065)

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STJ114065
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Host: Rabbit
Applications: WB/ELISA
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Clonality : Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3.
Purification: Affinity purification
Concentration: Lot specific
Dilution Range: WB:1:500-1:2000
ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PPIH
Gene ID: 10465
Uniprot ID: PPIH_HUMAN
Immunogen Region: 1-177
Specificity: Recombinant fusion protein containing a sequence corresponding to amino acids 1-177 of human PPIH (NP_006338.1).
Immunogen Sequence: MAVANSSPVNPVVFFDVSIG GQEVGRMKIELFADVVPKTA ENFRQFCTGEFRKDGVPIGY KGSTFHRVIKDFMIQGGDFV NGDGTGVASIYRGPFADENF KLRHSAPGLLSMANSGPSTN GCQFFITCSKCDWLDGKHVV FGKIIDGLLVMRKIENVPTG PNNKPKLPVVISQCGEM
Function PPIase that catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may therefore assist protein folding. Participates in pre-mRNA splicing. May play a role in the assembly of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. May act as a chaperone.
Protein Name Peptidyl-Prolyl Cis-Trans Isomerase H
Ppiase H
Rotamase H
Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H
Cyph
U-Snrnp-Associated Cyclophilin Snucyp-20
Usa-Cyp
Database Links Reactome: R-HSA-72163
Reactome: R-HSA-9692916
Cellular Localisation Nucleus Speckle
Cytoplasm
Colocalizes With Spliceosomal Snrnps
A Small Proportion May Also Be Cytoplasmic
Alternative Antibody Names Anti-Peptidyl-Prolyl Cis-Trans Isomerase H antibody
Anti-Ppiase H antibody
Anti-Rotamase H antibody
Anti-Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H antibody
Anti-Cyph antibody
Anti-U-Snrnp-Associated Cyclophilin Snucyp-20 antibody
Anti-Usa-Cyp antibody
Anti-PPIH antibody
Anti-CYP20 antibody
Anti-CYPH antibody

Information sourced from Uniprot.org

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