Anti-PPAP2A antibody (1-100) (STJ25067)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal PPAP2A (1-100) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | PLPP1 |
| Gene ID | 8611 |
| Uniprot ID | PLPP1_HUMAN |
| Immunogen Region | 1-100 |
| Immunogen Sequence | MFDKTRLPYVALDVLCVLLA GLPFAILTSRHTPFQRGVFC NDESIKYPYKEDTIPYALLG GIIIPFSIIVIILGETLSVY CNLLHSNSFIRNNYIATIYK |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human PPAP2A (NP_003702.2). |
Additional Info
| Tissue Specificity | Widely expressed with highest expression found in prostate. Found to be down-regulated in colon adenocarcinomas. Isoform 1: Predominant in kidney, lung, placenta and liver. Isoform 2: Predominant in heart and pancreas. |
| Post Translational Modifications | N-glycosylated. N-linked sugars are of the complex type. N-glycosylation is not required for the phosphatase activity. |
| Function | Magnesium-independent phospholipid phosphatase of the plasma membrane that catalyzes the dephosphorylation of a variety of glycerolipid and sphingolipid phosphate esters including phosphatidate/PA, lysophosphatidate/LPA, diacylglycerol pyrophosphate/DGPP, sphingosine 1-phosphate/S1P and ceramide 1-phosphate/C1P. Also acts on N-oleoyl ethanolamine phosphate/N-(9Z-octadecenoyl)-ethanolamine phosphate, a potential physiological compound. Through its extracellular phosphatase activity allows both the hydrolysis and the cellular uptake of these bioactive lipid mediators from the milieu, regulating signal transduction in different cellular processes. It is for instance essential for the extracellular hydrolysis of S1P and subsequent conversion into intracellular S1P. Involved in the regulation of inflammation, platelets activation, cell proliferation and migration among other processes. May also have an intracellular activity to regulate phospholipid-mediated signaling pathways. |
| Protein Name | Phospholipid Phosphatase 1 Lipid Phosphate Phosphohydrolase 1Pap2-AlphaPhosphatidate Phosphohydrolase Type 2aPhosphatidic Acid Phosphatase 2aPap-2aPap2a |
| Database Links | Reactome: R-HSA-9845614 |
| Cellular Localisation | Cell MembraneMulti-Pass Membrane ProteinApical Cell MembraneMembrane RaftMembraneCaveola |
| Alternative Antibody Names | Anti-Phospholipid Phosphatase 1 antibodyAnti-Lipid Phosphate Phosphohydrolase 1 antibodyAnti-Pap2-Alpha antibodyAnti-Phosphatidate Phosphohydrolase Type 2a antibodyAnti-Phosphatidic Acid Phosphatase 2a antibodyAnti-Pap-2a antibodyAnti-Pap2a antibodyAnti-PLPP1 antibodyAnti-LPP1 antibodyAnti-PPAP2A antibody |
Information sourced from Uniprot.org