• Western blot analysis of extracts of various cell lines, using POU2F2 antibody (STJ11104267) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-POU2F2 antibody (400-479) (STJ11104267)

SKU:
STJ11104267

£112.50 - £522.50
Current Stock:
Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Pou Domain-Class 2-Transcription Factor 2 (400-479) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2F2
Gene ID: 5452
Uniprot ID: PO2F2_HUMAN
Immunogen Region: 400-479
Immunogen: Recombinant fusion protein containing a sequence within amino acids 400-479 of human POU2F2 (NP_001193954.1).
Immunogen Sequence: VTTLSSAVGTLHPSRTAGGG GGGGGAAPPLNSIPSVTPPP PATTNSTNPSPQGSHSAIGL SGLNPSTGPGLWWNPAPYQP
Tissue Specificity Isoform 3 is B-cell specific. Isoform 5 is expressed in B-cells and the immunoglobulin-expressing T-cell line MOLT-4, but not in the T-cell line BW5147.
Function Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates IL6 expression in B cells with POU2AF1. Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR. Isoform 5: Activates the U2 small nuclear RNA (snRNA) promoter.
Protein Name Pou Domain - Class 2 - Transcription Factor 2
Lymphoid-Restricted Immunoglobulin Octamer-Binding Protein Nf-A2
Octamer-Binding Protein 2
Oct-2
Octamer-Binding Transcription Factor 2
Otf-2
Database Links Reactome: R-HSA-6807505
Cellular Localisation Cytoplasm
Nucleus
Alternative Antibody Names Anti-Pou Domain - Class 2 - Transcription Factor 2 antibody
Anti-Lymphoid-Restricted Immunoglobulin Octamer-Binding Protein Nf-A2 antibody
Anti-Octamer-Binding Protein 2 antibody
Anti-Oct-2 antibody
Anti-Octamer-Binding Transcription Factor 2 antibody
Anti-Otf-2 antibody
Anti-POU2F2 antibody
Anti-OCT2 antibody
Anti-OTF2 antibody

Information sourced from Uniprot.org

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