• HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human POU2AF1 with DYKDDDDK tag probed with STJ72228 (0. 5ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/3000) in lane C. Mock-transfected HEK293 probed with STJ72228 (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
  • STJ72228 (0. 3µg/ml) staining of Daudi lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-POU2AF1 antibody (Internal) (STJ72228)

SKU:
STJ72228-100

£303.50
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Host: Goat
Applications: Pep-ELISA/WB
Reactivity: Human/Mouse/Rat/Dog/Cow
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-POU2AF1 (Internal) is suitable for use in ELISA and Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration: 0.5 mg/mL
Dilution Range: ELISA-antibody detection limit dilution 1:32000.
Storage Instruction: Store at-20°C on receipt and minimise freeze-thaw cycles.
Gene Symbol: POU2AF1
Gene ID: 5450
Uniprot ID: OBF1_HUMAN
Immunogen Region: Internal
Accession Number: NP_006226.2
Immunogen Sequence: YTEYVPHEAVSCP
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
Protein Name Pou Domain Class 2-Associating Factor 1
B-Cell-Specific Coactivator Obf-1
Bob-1
Oca-B
Oct-Binding Factor 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain Class 2-Associating Factor 1 antibody
Anti-B-Cell-Specific Coactivator Obf-1 antibody
Anti-Bob-1 antibody
Anti-Oca-B antibody
Anti-Oct-Binding Factor 1 antibody
Anti-POU2AF1 antibody
Anti-BOB1 antibody
Anti-OBF1 antibody

Information sourced from Uniprot.org

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