| Short Description : | Goat polyclonal antibody anti-POU2AF1 (C-Term) is suitable for use in ELISA, Western Blot and Immunohistochemistry research applications. |
| Applications: | Pep-ELISA/WB-Trf/IHC |
| Host: | Goat |
| Reactivity: | Human/Dog/Cow |
| Note: | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Clonality : | Polyclonal |
| Isotype: | IgG |
| Conjugation: | Unconjugated |
| Concentration: | 0.5 mg/mL |
| Purification: | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Dilution Range: | Peptide ELISA: antibody detection limit dilution 1:8000.WB: In transfected HEK293 transiently expressing full-length Human POU2AF1 (myc and DYKDDDDK tagged) , a band of approx. 38kDa was observed. No bands were observed in mock-transfected HEK29 |
| Formulation: | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA |
| Storage Instruction: | Store at-20°C on receipt and minimise freeze-thaw cycles. |
| Gene Symbol: | POU2AF1 |
| Gene ID: | 5450 |
| Uniprot ID: | OBF1_HUMAN |
| Accession Number: | NP_006226.2 |
| Immunogen Region: | C-Term |
| Immunogen Sequence: | DSDAYALNHTLSVE |
| Post Translational Modifications | Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation. |
| Function | Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator. |
| Protein Name | Pou Domain Class 2-Associating Factor 1B-Cell-Specific Coactivator Obf-1Bob-1Oca-BOct-Binding Factor 1 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Pou Domain Class 2-Associating Factor 1 antibodyAnti-B-Cell-Specific Coactivator Obf-1 antibodyAnti-Bob-1 antibodyAnti-Oca-B antibodyAnti-Oct-Binding Factor 1 antibodyAnti-POU2AF1 antibodyAnti-BOB1 antibodyAnti-OBF1 antibody |
Information sourced from Uniprot.org
