• Immunohistochemical analysis of paraffin-embedded Human spleen. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
  • Immunohistochemical analysis of paraffin-embedded human-colon, antibody was diluted at 1:200

Anti-POU2AF1 antibody (201-250 aa) (STJ98803)

SKU:
STJ98803

£46.00 - £226.00
Current Stock:
Host: Rabbit
Applications: IHC/IF/ELISA
Reactivity: Human/Rat/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-POU domain class 2-associating factor 1 (201-250 aa) is suitable for use in Immunohistochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IHC-P 1:50-200
ELISA 1:10000-20000
IF 1:50-200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: POU2AF1
Gene ID: 5450
Uniprot ID: OBF1_HUMAN
Immunogen Region: 201-250 aa
Specificity: The antibody detects endogenous Bob 1
Immunogen: Synthetic peptide from the human protein at the amino acid range 201-250
Post Translational Modifications Ubiquitinated.mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
Function Transcriptional coactivator that specifically associates with either POU2F1/OCT1 or POU2F2/OCT2. It boosts the POU2F1/OCT1 mediated promoter activity and to a lesser extent, that of POU2F2/OCT2. It recognizes the POU domains of POU2F1/OCT1 and POU2F2/OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. Regulates IL6 expression in B cells as POU2F2/OCT2 coactivator.
Protein Name Pou Domain Class 2-Associating Factor 1
B-Cell-Specific Coactivator Obf-1
Bob-1
Oca-B
Oct-Binding Factor 1
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Pou Domain Class 2-Associating Factor 1 antibody
Anti-B-Cell-Specific Coactivator Obf-1 antibody
Anti-Bob-1 antibody
Anti-Oca-B antibody
Anti-Oct-Binding Factor 1 antibody
Anti-POU2AF1 antibody
Anti-BOB1 antibody
Anti-OBF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance