Anti-POMK antibody (1-350) (STJ117724)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal POMK (1-350) antibody for use in WB and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:200-1:2000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POMK |
| Gene ID | 84197 |
| Uniprot ID | SG196_HUMAN |
| Immunogen Region | 1-350 |
| Immunogen Sequence | MEKQPQNSRRGLAPREVPPA VGLLLIMALMNTLLYLCLDH FFIAPRQSTVDPTHCPYGHF RIGQMKNCSPWLSCEELRTE VRQLKRVGEGAVKRVFLSEW KEHKVALSQLTSLEMKDDFL HGLQMLKSLQGTHVVTLLGY CEDDNTMLTEYHPLGSLSNL EETLNLSKYQNVNTWQHRLE LAMDYVSIINYLHHSPVGTR VMCDSNDLPKTLSQYLLTSN FSILANDLDALPLVNHSSG |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human POMK (NP_115613.1). |
Additional Info
| Tissue Specificity | Highest expression is observed in brain, skeletal muscle, kidney and heart in fetal and adult tissues. |
| Function | Protein O-mannose kinase that specifically mediates phosphorylation at the 6-position of an O-mannose of the trisaccharide (N-acetylgalactosamine (GalNAc)-beta-1,3-N-acetylglucosamine (GlcNAc)-beta-1,4-mannose) to generate phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-1,3-N-acetylglucosamine-beta-1,4-(phosphate-6-)mannose). Phosphorylated O-mannosyl trisaccharide is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity. Only shows kinase activity when the GalNAc-beta-3-GlcNAc-beta-terminus is linked to the 4-position of O-mannose, suggesting that this disaccharide serves as the substrate recognition motif. |
| Protein Name | Protein O-Mannose KinasePomkProtein Kinase-Like Protein Sgk196Sugen Kinase 196 |
| Database Links | Reactome: R-HSA-5173105 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Type Ii Membrane Protein |
| Alternative Antibody Names | Anti-Protein O-Mannose Kinase antibodyAnti-Pomk antibodyAnti-Protein Kinase-Like Protein Sgk196 antibodyAnti-Sugen Kinase 196 antibodyAnti-POMK antibodyAnti-SGK196 antibody |
Information sourced from Uniprot.org