Anti-POLR3D antibody (281-330 aa) (STJ95181)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human RPC4 at the amino acid range 281-330
General Information
| Short Description | Rabbit polyclonal anti-DNA-directed RNA polymerase III subunit RPC4 (281-330 aa) for use in WB, ELISA and IHC in Human and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/IHC |
| Host | Rabbit |
| Reactivity | Human/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000IHC-P 1:50-300ELISA 2000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLR3D |
| Gene ID | 661 |
| Uniprot ID | RPC4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human RPC4 at the amino acid range 281-330 |
| Immunogen Region | 281-330 aa |
| Specificity | POLR3D Polyclonal Antibody detects endogenous levels of POLR3D protein. |
Additional Info
| Function | DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Assembles with POLR3E/RPC5 forming a subcomplex that binds the Pol III core. Enables recruitment of Pol III at transcription initiation site and drives transcription initiation from both type 2 and type 3 DNA promoters. Required for efficient transcription termination and reinitiation. Pol III plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-kappa-B through the RIG-I pathway. |
| Protein Name | Dna-Directed Rna Polymerase Iii Subunit Rpc4Rna Polymerase Iii Subunit C4Dna-Directed Rna Polymerase Iii Subunit DProtein Bn51Rna Polymerase Iii 47 Kda SubunitRpc53 Homolog |
| Database Links | Reactome: R-HSA-1834949Reactome: R-HSA-73780Reactome: R-HSA-73980Reactome: R-HSA-749476Reactome: R-HSA-76061Reactome: R-HSA-76066Reactome: R-HSA-76071 |
| Cellular Localisation | Nucleus |
| Alternative Antibody Names | Anti-Dna-Directed Rna Polymerase Iii Subunit Rpc4 antibodyAnti-Rna Polymerase Iii Subunit C4 antibodyAnti-Dna-Directed Rna Polymerase Iii Subunit D antibodyAnti-Protein Bn51 antibodyAnti-Rna Polymerase Iii 47 Kda Subunit antibodyAnti-Rpc53 Homolog antibodyAnti-POLR3D antibodyAnti-BN51 antibodyAnti-BN51T antibody |
Information sourced from Uniprot.org