Anti-POLI antibody (641-690 aa) (STJ92742)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human POLI at the amino acid range 641-690
General Information
| Short Description | Rabbit polyclonal anti-DNA polymerase iota (641-690 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000ELISA 1:40000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLI |
| Gene ID | 11201 |
| Uniprot ID | POLI_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human POLI at the amino acid range 641-690 |
| Immunogen Region | 641-690 aa |
| Specificity | DNA pol Iota Polyclonal Antibody detects endogenous levels of DNA pol Iota protein. |
Additional Info
| Post Translational Modifications | Monoubiquitinated. Protein monoubiquitination prevents POLI binding to ubiquitin via the ubiquitin-binding motif 1 and ubiquitin-binding motif 2. |
| Function | Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls. Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high-fidelity opposite an adenosine template. Exhibits low fidelity and efficiency opposite a thymidine template, where it will preferentially insert guanosine. May play a role in hypermutation of immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity. |
| Protein Name | Dna Polymerase IotaEta2Rad30 Homolog B |
| Database Links | Reactome: R-HSA-5656121Reactome: R-HSA-5656169 |
| Cellular Localisation | NucleusBinding To Ubiquitin Mediates Localization To Replication Forks After Uv-Induced Dna Damage |
| Alternative Antibody Names | Anti-Dna Polymerase Iota antibodyAnti-Eta2 antibodyAnti-Rad30 Homolog B antibodyAnti-POLI antibodyAnti-RAD30B antibody |
Information sourced from Uniprot.org