Anti-POLB antibody (286-335 aa) (STJ92734)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from the human DNA Polymerase beta at the amino acid range 286-335
General Information
| Short Description | Rabbit polyclonal anti-DNA polymerase beta (286-335 aa) for use in WB, IHC, IF and ELISA in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-1:2000IHC 1:100-1:300ELISA 1:10000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | POLB |
| Gene ID | 5423 |
| Uniprot ID | DPOLB_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from the human DNA Polymerase beta at the amino acid range 286-335 |
| Immunogen Region | 286-335 aa |
| Specificity | DNA pol Beta Polyclonal Antibody detects endogenous levels of DNA pol Beta protein. |
Additional Info
| Function | Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase).through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. |
| Protein Name | Dna Polymerase Beta5'-Deoxyribose-Phosphate Lyase5'-Drp LyaseAp Lyase |
| Database Links | Reactome: R-HSA-110362Reactome: R-HSA-110373Reactome: R-HSA-110381Reactome: R-HSA-5649702Reactome: R-HSA-5651801Reactome: R-HSA-5689880Reactome: R-HSA-73930 |
| Cellular Localisation | NucleusCytoplasmCytoplasmic In Normal ConditionsTranslocates To The Nucleus Following Dna Damage |
| Alternative Antibody Names | Anti-Dna Polymerase Beta antibodyAnti-5'-Deoxyribose-Phosphate Lyase antibodyAnti-5'-Drp Lyase antibodyAnti-Ap Lyase antibodyAnti-POLB antibody |
Information sourced from Uniprot.org